LOCUS       sequence-72794-e         863 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Destination dual luciferase reporter plasmid for checking 
            effectiveness of shRNA. Compatible with standard AttL1-AttL2 Gateway
            Entry vectors..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 863)
  AUTHORS   Geiling B, Vandal G, Posner AR, de Bruyns A, Dutchak KL, Garnett S, 
            Dankort D
  TITLE     A modular lentiviral and retroviral construction system to rapidly 
            generate vectors for gene expression and gene knockdown in vitro and
            in vivo.
  JOURNAL   PLoS One. 2013 Oct 11;8(10):e76279. doi: 
            10.1371/journal.pone.0076279.
  PUBMED    24146852
REFERENCE   2  (bases 1 to 863)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2013-10-11"; volume: "8(10):e76279. doi"; pages: " 
            10.1371/journal.pone.0076279"
COMMENT     Sequence Label: pCheck2 Dest (R1-R2)   #BGV129
FEATURES             Location/Qualifiers
     source          1..863
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    198..322
                     /gene="mutant version of attR"
                     /label=attR1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     promoter        join(351..356,357..374,375..381)
                     /label=lac UV5 promoter
                     /note="E. coli lac promoter with an ""up"" mutation"
     primer_bind     complement(501..520)
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
ORIGIN
        1 gttcatcgag tccgaccctg ggttcttttc caacgctatt gtcgagggag ctaagaagtt
       61 ccctaacacc gagttcgtga aggtgaaggg cctccacttc agccaggagg acgctccaga
      121 tgaaatgggt aagtacatca agagcttcgt ggagcgcgtg ctgaagaacg agcagtaatt
      181 ctaggcgatc gctcgagaca agtttgtaca aaaaagctga acgagaaacg taaaatgata
      241 taaatatcaa tatattaaat tagattttgc ataaaaaaca gactacataa tactgtaaaa
      301 cacaacatat ccagtcacta tggcggccgg ccgcattagg caccccaggc tttacacttt
      361 atgcttccgg ctcgtataat gtgtggattt tgagttagga tccgtcgaga ttttcaggag
      421 ctaaggaagc taaaatggag aaaaaaatca ctggatatac caccgttgat atatcccaat
      481 ggcatcgtaa agaacatttt gaggcatttc agtcagttgc tcaatgtacc tataaccaga
      541 ccgttcagct ggatattacg gcctttttaa agaccgtaaa gaaaaataag cacaagtttt
      601 atccggcctt tattcacatt cttgcccgcc tgatgaatgc tcatccggaa ttccgtatgg
      661 caatgaaaga cggtgagctg gtgatatggg atagtgttca cccttgttac accgttttcc
      721 atgagcaaac tgaaacgttt tcatcgctct ggagtgaata ccacgacgat ttccggcagt
      781 ttctacacat atattcgcaa gatgtggcgt gttacggtga aaacctggcc tatttcccta
      841 aagggtttat tgagaatatg ttt
//
