LOCUS       sequence-72797-e         954 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral Destination vector containing AttR1-AttR3 site. For use 
            with three-way LR reaction with cDNA in entry vector and a marker in
            pBEG R2-L3 plasmids (module 2 and module 3 plasmids)..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 954)
  AUTHORS   Geiling B, Vandal G, Posner AR, de Bruyns A, Dutchak KL, Garnett S, 
            Dankort D
  TITLE     A modular lentiviral and retroviral construction system to rapidly 
            generate vectors for gene expression and gene knockdown in vitro and
            in vivo.
  JOURNAL   PLoS One. 2013 Oct 11;8(10):e76279. doi: 
            10.1371/journal.pone.0076279.
  PUBMED    24146852
REFERENCE   2  (bases 1 to 954)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2013-10-11"; volume: "8(10):e76279. doi"; pages: " 
            10.1371/journal.pone.0076279"
COMMENT     Sequence Label: pLEG(R1-R3)   #BGV149
FEATURES             Location/Qualifiers
     source          1..954
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    86..210
                     /gene="mutant version of attR"
                     /label=attR1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     promoter        join(241..246,247..264,265..271)
                     /label=lac UV5 promoter
                     /note="E. coli lac promoter with an ""up"" mutation"
     primer_bind     complement(391..410)
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
ORIGIN
        1 gtgaaccgtc agatcgcctg gagacgccat ccacgctgtt ttgacctcca tagaagacac
       61 cgactctact agaggatctg ctagcacaag tttgtacaaa aaagctgaac gagaaacgta
      121 aaatgatata aatatcaata tattaaatta gattttgcat aaaaaacaga ctacataata
      181 ctgtaaaaca caacatatcc agtcactatg gccggcgcgg ccgcattagg caccccaggc
      241 tttacacttt atgcttccgg ctcgtataat gtgtggattt tgagttagga tccgtcgaga
      301 ttttcaggag ctaaggaagc taaaatggag aaaaaaatca ctggatatac caccgttgat
      361 atatcccaat ggcatcgtaa agaacatttt gaggcatttc agtcagttgc tcaatgtacc
      421 tataaccaga ccgttcagct ggatattacg gcctttttaa agaccgtaaa gaaaaataag
      481 cacaagtttt atccggcctt tattcacatt cttgcccgcc tgatgaatgc tcatccggaa
      541 ttccgtatgg caatgaaaga cggtgagctg gtgatatggg atagtgttca cccttgttac
      601 accgttttcc atgagcaaac tgaaacgttt tcatcgctct ggagtgaata ccacgacgat
      661 ttccggcagt ttctacacat atattcgcaa gatgtggcgt gttacggtga aaacctggcc
      721 tatttcccta aagggtttat tgagaatatg tttttcgtct cagccaatcc ctgggtgagt
      781 ttcaccagtt ttgatttaaa cgtggccaat atggacaact tcttcgcccc cgttttcacc
      841 atgggcaaat attatacgca aggcgacaag gtgctgatgc cgctggcgat tcaggttcat
      901 catgccgttt gtgatggctt ccatgtcggg cagaatgctt aatgaattac aaca
//
