LOCUS       sequence-72799-e         913 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral Destination vector containing AttR1-AttR4 site. Use with 
            4-way LR reaction with cDNA in entry vector, marker in pBEG R2-L3 
            plasmids and shRNA in pBEG R3-L4 plasmid (module 2,3,and 4) .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 913)
  AUTHORS   Geiling B, Vandal G, Posner AR, de Bruyns A, Dutchak KL, Garnett S, 
            Dankort D
  TITLE     A modular lentiviral and retroviral construction system to rapidly 
            generate vectors for gene expression and gene knockdown in vitro and
            in vivo.
  JOURNAL   PLoS One. 2013 Oct 11;8(10):e76279. doi: 
            10.1371/journal.pone.0076279.
  PUBMED    24146852
REFERENCE   2  (bases 1 to 913)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2013-10-11"; volume: "8(10):e76279. doi"; pages: " 
            10.1371/journal.pone.0076279"
COMMENT     Sequence Label: pLEG(R1-R4)   #BGV057
FEATURES             Location/Qualifiers
     source          1..913
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    82..206
                     /gene="mutant version of attR"
                     /label=attR1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     promoter        join(237..242,243..260,261..267)
                     /label=lac UV5 promoter
                     /note="E. coli lac promoter with an ""up"" mutation"
     primer_bind     complement(387..406)
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
ORIGIN
        1 accgtcagat cgcctggaga cgccatccac gctgttttga cctccataga agacaccgac
       61 tctactagag gatctgctag cacaagtttg tacaaaaaag ctgaacgaga aacgtaaaat
      121 gatataaata tcaatatatt aaattagatt ttgcataaaa aacagactac ataatactgt
      181 aaaacacaac atatccagtc actatggccg gcgcggccgc attaggcacc ccaggcttta
      241 cactttatgc ttccggctcg tataatgtgt ggattttgag ttaggatccg tcgagatttt
      301 caggagctaa ggaagctaaa atggagaaaa aaatcactgg atataccacc gttgatatat
      361 cccaatggca tcgtaaagaa cattttgagg catttcagtc agttgctcaa tgtacctata
      421 accagaccgt tcagctggat attacggcct ttttaaagac cgtaaagaaa aataagcaca
      481 agttttatcc ggcctttatt cacattcttg cccgcctgat gaatgctcat ccggaattcc
      541 gtatggcaat gaaagacggt gagctggtga tatgggatag tgttcaccct tgttacaccg
      601 ttttccatga gcaaactgaa acgttttcat cgctctggag tgaataccac gacgatttcc
      661 ggcagtttct acacatatat tcgcaagatg tggcgtgtta cggtgaaaac ctggcctatt
      721 tccctaaagg gtttattgag aatatgtttt tcgtctcagc caatccctgg gtgagtttca
      781 ccagttttga tttaaacgtg gccaatatgg acaacttctt cgcccccgtt ttcaccatgg
      841 gcaaatatta tacgcaaggc gacaaggtgc tgatgccgct ggcgattcag gttcatcatg
      901 ccgtttgtga tgg
//
