LOCUS       sequence-72807-e        1150 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  encodes enhanced green fluorescent protein (EGFP).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1150)
  TITLE     pSG5-EGFP
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1150)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pSG5-EGFP
FEATURES             Location/Qualifiers
     source          1..1150
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(35..56)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(296..315)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     643..664
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     polyA_signal    736..857
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(773..792)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     827..846
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(891..908)
                     /label=L4440
                     /note="L4440 vector, forward primer"
ORIGIN
        1 gnncgaggag ctgttcaccg gggtggtgcc catcctggtc gagctggacg gcgacgtaaa
       61 cggccacaag ttcagcgtgt ccggcgaggg cgagggcgat gccacctacg gcaagctgac
      121 cctgaagttc atctgcacca ccggcaagct gcccgtgccc tggcccaccc tcgtgaccac
      181 cctgacctac ggcgtgcagt gcttcagccg ctaccccgac cgcatgaagc agcacgactt
      241 cttcaagtcc gccatgcccg aaggctacgt ccaggagcgc accatcttct tcaaggacga
      301 cggcaactac aagacccgcg ccgaggtgaa gttcgagggc gacaccctgg tgaaccgcat
      361 cgagctgaag ggcatcgact tcaaggagga cggcaacatc ctggggcaca agctggagta
      421 caactacaac agccacaacg tctatatcat ggccgacaag cagaagaacg gcatcaaggt
      481 gaacttcaag atccgccaca acatcgagga cggcagcgtg cagctcgccg accactacca
      541 gcagaacacc cccattggcg acggccccgt gctgctgccc gacaaccact acctgagcac
      601 ccagtccgcc ctgagcaaag accccaacga gaagcgcgat cacatggtcc tgctggagtt
      661 cgtgaccgcc gccgggatca ctctcggcat ggacgagctg tacaagtact gaggatccag
      721 atcttattaa agcagaactt gtttattgca gcttataatg gttacaaata aagcaatagc
      781 atcacaaatt tcacaaataa agcatttttt tcactgcatt ctagttgtgg tttgtccaaa
      841 ctcatcaatg tatcttatca tgtctggtcg actctagact cttccgcttc ctcgctcact
      901 gactcgctgc gctcggtcgt tcggctgcgg cgagcggtat cagctcactc aangcggtaa
      961 tannnttatc cacagaatcn gggataacgc nnnaananat gtgagcaaaa ggccagcaaa
     1021 agncagnanc gtaaaannng cgttgctngc gtttttcnnn gctcnncccc tgacgancat
     1081 cncaaaatcn annctcagtc nnagnnnnaa nccgnnnnnn tnnnagannc cagnnttccc
     1141 cnnnnctncc
//
