LOCUS       sequence-72809-e         934 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Retroviral Destination vector containing AttR1-AttR3 site. For use 
            with three-way LR reaction with cDNA in entry vector and a marker in
            pBEG R2-L3 plasmids (module 2 and module 3 plasmids)..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 934)
  AUTHORS   Geiling B, Vandal G, Posner AR, de Bruyns A, Dutchak KL, Garnett S, 
            Dankort D
  TITLE     A modular lentiviral and retroviral construction system to rapidly 
            generate vectors for gene expression and gene knockdown in vitro and
            in vivo.
  JOURNAL   PLoS One. 2013 Oct 11;8(10):e76279. doi: 
            10.1371/journal.pone.0076279.
  PUBMED    24146852
REFERENCE   2  (bases 1 to 934)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2013-10-11"; volume: "8(10):e76279. doi"; pages: " 
            10.1371/journal.pone.0076279"
COMMENT     Sequence Label: pREG(R1-R3)   #BGV141
FEATURES             Location/Qualifiers
     source          1..934
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    96..220
                     /gene="mutant version of attR"
                     /label=attR1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     promoter        join(245..250,251..268,269..275)
                     /label=lac UV5 promoter
                     /note="E. coli lac promoter with an ""up"" mutation"
     primer_bind     complement(395..414)
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
ORIGIN
        1 ttgacctcca tagaagacac cgggaccgat ccagcctccg cggccgggaa cggtgcattg
       61 gaacggacct gcaggaattg atccgcggcc atcaaacaag tttgtacaaa aaagctgaac
      121 gagaaacgta aaatgatata aatatcaata tattaaatta gattttgcat aaaaaacaga
      181 ctacataata ctgtaaaaca caacatatcc agtcactatg gcggccgcat taggcacccc
      241 aggctttaca ctttatgctt ccggctcgta taatgtgtgg attttgagtt aggatccgtc
      301 gagattttca ggagctaagg aagctaaaat ggagaaaaaa atcactggat ataccaccgt
      361 tgatatatcc caatggcatc gtaaagaaca ttttgaggca tttcagtcag ttgctcaatg
      421 tacctataac cagaccgttc agctggatat tacggccttt ttaaagaccg taaagaaaaa
      481 taagcacaag ttttatccgg cctttattca cattcttgcc cgcctgatga atgctcatcc
      541 ggaattccgt atggcaatga aagacggtga gctggtgata tgggatagtg ttcacccttg
      601 ttacaccgtt ttccatgagc aaactgaaac gttttcatcg ctctggagtg aataccacga
      661 cgatttccgg cagtttctac acatatattc gcaagatgtg gcgtgttacg gtgaaaacct
      721 ggcctatttc cctaaagggt ttattgagaa tatgtttttc gtctcagcca atccctgggt
      781 gagtttcacc agttttgatt taaacgtggc caatatggac aacttcttcg cccccgtttt
      841 caccatgggc aaatattata cgcaaggcga caaggtgctg atgccgctgg cgattcaggt
      901 tcatcatgcc gtttgtgatg gcttccatgt cggc
//
