LOCUS       sequence-7281-ex       10714 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10714)
  TITLE     pBOB plasmids
REFERENCE   2  (bases 1 to 10714)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pBOB-CAG-iCRE-SD
FEATURES             Location/Qualifiers
     source          1..10714
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(44..63)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        235..614
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        616..814
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     769..789
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     LTR             832..1012
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    1056..1181
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1674..1907
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             2092..2136
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2388..2505
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     enhancer        2515..2894
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        2897..3172
                     /label=chicken beta-actin promoter
     intron          3173..4181
                     /label=chimeric intron
                     /note="chimera between introns from chicken beta-actin and 
                     rabbit beta-globin"
     primer_bind     4105..4127
                     /label=pCAGGS-5
                     /note="Chimeric intron in CAG promoter, forward primer"
     CDS             4291..4311
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     misc_feature    5480..6068
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(5533..5553)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(5951..5962)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     protein_bind    complement(6468..6501)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     LTR             6515..6695
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(6721..6738)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    6727..6951
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      6997..7425
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(7084..7103)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     7294..7315
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(7434..7454)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        7439..7769
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      7620..7755
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     7682..7701
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     promoter        7817..7864
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             7883..8257
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     polyA_signal    8387..8508
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(8424..8443)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     8478..8497
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(8557..8573)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(8557..8573)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(8570..8592)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    8581..8597
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(8605..8611,8612..8629,8630..8635))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    8650..8671
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(8788..8805)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(8959..9547)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(9039..9058)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(9718..10509,10510..10578))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     10341..10360
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(10579..10683)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 gacggatcgg gagatctccc gatcccctat ggtcgactct cagtacaatc tgctctgatg
       61 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg
      121 cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc
      181 ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgttgacatt
      241 gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata
      301 tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc
      361 cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc
      421 attgacgtca atgggtggac tatttacggt aaactgccca cttggcagta catcaagtgt
      481 atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt
      541 atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca
      601 tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg
      661 actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc
      721 aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg
      781 gtaggcgtgt acggtgggag gtctatataa gcagcgcgtt ttgcctgtac tgggtctctc
      841 tggttagacc agatctgagc ctgggagctc tctggctaac tagggaaccc actgcttaag
      901 cctcaataaa gcttgccttg agtgcttcaa gtagtgtgtg cccgtctgtt gtgtgactct
      961 ggtaactaga gatccctcag acccttttag tcagtgtgga aaatctctag cagtggcgcc
     1021 cgaacaggga cctgaaagcg aaagggaaac cagagctctc tcgacgcagg actcggcttg
     1081 ctgaagcgcg cacggcaaga ggcgaggggc ggcgactggt gagtacgcca aaaattttga
     1141 ctagcggagg ctagaaggag agagatgggt gcgagagcgt cagtattaag cgggggagaa
     1201 ttagatcgcg atgggaaaaa attcggttaa ggccaggggg aaagaaaaaa tataaattaa
     1261 aacatatagt atgggcaagc agggagctag aacgattcgc agttaatcct ggcctgttag
     1321 aaacatcaga aggctgtaga caaatactgg gacagctaca accatccctt cagacaggat
     1381 cagaagaact tagatcatta tataatacag tagcaaccct ctattgtgtg catcaaagga
     1441 tagagataaa agacaccaag gaagctttag acaagataga ggaagagcaa aacaaaagta
     1501 agaccaccgc acagcaagcg gccgctgatc ttcagacttg gaggaggaga tatgagggac
     1561 aattggagaa gtgaattata taaatataaa gtagtaaaaa ttgaaccatt aggagtagca
     1621 cccaccaagg caaagagaag agtggtgcag agagaaaaaa gagcagtggg aataggagct
     1681 ttgttccttg ggttcttggg agcagcagga agcactatgg gcgcagcctc aatgacgctg
     1741 acggtacagg ccagacaatt attgtctggt atagtgcagc agcagaacaa tttgctgagg
     1801 gctattgagg cgcaacagca tctgttgcaa ctcacagtct ggggcatcaa gcagctccaa
     1861 gcaagaatcc tagctgtgga aagataccta aaggatcaac agctcctagg gatttggggt
     1921 tgctctggaa aactcatttg caccactgct gtgccttgga atgctagttg gagtaataaa
     1981 tctctggaac agatctggaa tcacacgacc tggatggagt gggacagaga aattaacaat
     2041 tacacaagct taatacactc cttaattgaa gaatcgcaaa accagcaaga aaagaatgaa
     2101 caagaattat tggaattaga taaatgggca agtttgtgga attggtttaa cataacaaat
     2161 tggctgtggt atataaaatt attcataatg atagtaggag gcttggtagg tttaagaata
     2221 gtttttgctg tactttctat agtgaataga gttaggcagg gatattcacc attatcgttt
     2281 cagacccacc tcccaatccc gaggggaccc gacaggcccg aaggaataga agaagaaggt
     2341 ggagagagag acagagacag atccattcga ttagtgaacg gatcaacttt taaaagaaaa
     2401 ggggggattg gggggtacag tgcaggggaa agaatagtag acataatagc aacagacata
     2461 caaactaaag aattacaaaa acaaattaca aaaattcaaa attttatcga tgtcgacatt
     2521 gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata
     2581 tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc
     2641 cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc
     2701 attgacgtca atgggtggac tatttacggt aaactgccca cttggcagta catcaagtgt
     2761 atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt
     2821 atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca
     2881 tcgctattac catgggtcga ggtgagcccc acgttctgct tcactctccc catctccccc
     2941 ccctccccac ccccaatttt gtatttattt attttttaat tattttgtgc agcgatgggg
     3001 gcgggggggg ggggggcgcg cgccaggcgg ggcggggcgg ggcgaggggc ggggcggggc
     3061 gaggcggaga ggtgcggcgg cagccaatca gagcggcgcg ctccgaaagt ttccttttat
     3121 ggcgaggcgg cggcggcggc ggccctataa aaagcgaagc gcgcggcggg cgggagtcgc
     3181 tgcgttgcct tcgccccgtg ccccgctccg cgccgcctcg cgccgcccgc cccggctctg
     3241 actgaccgcg ttactcccac aggtgagcgg gcgggacggc ccttctcctc cgggctgtaa
     3301 ttagcgcttg gtttaatgac ggctcgtttc ttttctgtgg ctgcgtgaaa gccttaaagg
     3361 gctccgggag ggccctttgt gcggggggga gcggctcggg gggtgcgtgc gtgtgtgtgt
     3421 gcgtggggag cgccgcgtgc ggcccgcgct gcccggcggc tgtgagcgct gcgggcgcgg
     3481 cgcggggctt tgtgcgctcc gcgtgtgcgc gaggggagcg cggccggggg cggtgccccg
     3541 cggtgcgggg gggctgcgag gggaacaaag gctgcgtgcg gggtgtgtgc gtgggggggt
     3601 gagcaggggg tgtgggcgcg gcggtcgggc tgtaaccccc ccctgcaccc ccctccccga
     3661 gttgctgagc acggcccggc ttcgggtgcg gggctccgtg cggggcgtgg cgcggggctc
     3721 gccgtgccgg gcggggggtg gcggcaggtg ggggtgccgg gcggggcggg gccgcctcgg
     3781 gccggggagg gctcggggga ggggcgcggc ggccccggag cgccggcggc tgtcgaggcg
     3841 cggcgagccg cagccattgc cttttatggt aatcgtgcga gagggcgcag ggacttcctt
     3901 tgtcccaaat ctggcggagc cgaaatctgg gaggcgccgc cgcaccccct ctagcgggcg
     3961 cgggcgaagc ggtgcggcgc cggcaggaag gaaatgggcg gggagggcct tcgtgcgtcg
     4021 ccgcgccgcc gtccccttct ccatctccag cctcggggct gccgcagggg gacggctgcc
     4081 ttcggggggg acggggcagg gcggggttcg gcttctggcg tgtgaccggc ggctctagag
     4141 cctctgctaa ccatgttcat gccttcttct ttttcctaca gctcctgggc aacgtgctgg
     4201 ttgttgtgct gtctcatcat tttggcaaag aattcgctag tggatccccc gggctgcagg
     4261 aattcgatat caagcttgtc caccatggtg cccaagaaga agaggaaagt ctccaacctg
     4321 ctgactgtgc accaaaacct gcctgccctc cctgtggatg ccacctctga tgaagtcagg
     4381 aagaacctga tggacatgtt cagggacagg caggccttct ctgaacacac ctggaagatg
     4441 ctcctgtctg tgtgcagatc ctgggctgcc tggtgcaagc tgaacaacag gaaatggttc
     4501 cctgctgaac ctgaggatgt gagggactac ctcctgtacc tgcaagccag aggcctggct
     4561 gtgaagacca tccaacagca cctgggccag ctcaacatgc tgcacaggag atctggcctg
     4621 cctcgccctt ctgactccaa tgctgtgtcc ctggtgatga ggagaatcag aaaggagaat
     4681 gtggatgctg gggagagagc caagcaggcc ctggcctttg aacgcactga ctttgaccaa
     4741 gtcagatccc tgatggagaa ctctgacaga tgccaggaca tcaggaacct ggccttcctg
     4801 ggcattgcct acaacaccct gctgcgcatt gccgaaattg ccagaatcag agtgaaggac
     4861 atctcccgca ccgatggtgg gagaatgctg atccacattg gcaggaccaa gaccctggtg
     4921 tccacagctg gtgtggagaa ggccctgtcc ctgggggtta ccaagctggt ggagagatgg
     4981 atctctgtgt ctggtgtggc tgatgacccc aacaactacc tgttctgccg ggtcagaaag
     5041 aatggtgtgg ctgccccttc tgccacctcc caactgtcca cccgggccct ggaagggatc
     5101 tttgaggcca cccaccgcct gatctatggt gccaaggatg actctgggca gagatacctg
     5161 gcctggtctg gccactctgc cagagtgggt gctgccaggg acatggccag ggctggtgtg
     5221 tccatccctg aaatcatgca ggctggtggc tggaccaatg tgaacattgt gatgaactac
     5281 atcagaaacc tggactctga gactggggcc atggtgaggc tgctcgagga tggggactga
     5341 aactgagtcg agggggggcc cgaaacctgg aaaaacatgg agcaatcaca agtagcaaca
     5401 cagcagctac caatgctgct tgtgcctggc tagaagcaca agaggaggag gaggtgggtt
     5461 ttccagtcac acctcagaca atcaacctct ggattacaaa atttgtgaaa gattgactgg
     5521 tattcttaac tatgttgctc cttttacgct atgtggatac gctgctttaa tgcctttgta
     5581 tcatgctatt gcttcccgta tggctttcat tttctcctcc ttgtataaat cctggttgct
     5641 gtctctttat gaggagttgt ggcccgttgt caggcaacgt ggcgtggtgt gcactgtgtt
     5701 tgctgacgca acccccactg gttggggcat tgccaccacc tgtcagctcc tttccgggac
     5761 tttcgctttc cccctcccta ttgccacggc ggaactcatc gccgcctgcc ttgcccgctg
     5821 ctggacaggg gctcggctgt tgggcactga caattccgtg gtgttgtcgg ggaagctgac
     5881 gtcctttcca tggctgctcg cctgtgttgc cacctggatt ctgcgcggga cgtccttctg
     5941 ctacgtccct tcggccctca atccagcgga ccttccttcc cgcggcctgc tgccggctct
     6001 gcggcctctt ccgcgtcttc gccttcgccc tcagacgagt cggatctccc tttgggccgc
     6061 ctccccgcct ggaattcgag ctcggtacct ttaagaccaa tgacttacaa ggcagctgta
     6121 gatcttagcc actttttaaa agaaaagggg ggactggaag ggctaattca ctcccaaaga
     6181 agacaagata tccttgatct gtggatctac cacacacaag gctacttccc tgattgacag
     6241 aactacacac cagggccagg ggtcagatat ccactgacct ttggatggtg ctacaagcta
     6301 gtaccagttg agccagataa gatagaagag gccaataaag gagagaacac cagcttgtta
     6361 caccctgtga gcctgcatgg gatggatgac ccggagagag aagtgttaga gtggaggttt
     6421 gacagccgcc tagcatttca tcacgtggcc cgagagctgc atccggcata acttcgtata
     6481 gcatacatta tacgaagtta tgccggactg tactgggtct ctctggttag accagatctg
     6541 agcctgggag ctctctggct aactagggaa cccactgctt aagcctcaat aaagcttgcc
     6601 ttgagtgctt caagtagtgt gtgcccgtct gttgtgtgac tctggtaact agagatccct
     6661 cagacccttt tagtcagtgt ggaaaatctc tagcagggcc gctttaaacc cgctgatcag
     6721 cctcgactgt gccttctagt tgccagccat ctgttgtttg cccctccccc gtgccttcct
     6781 tgaccctgga aggtgccact cccactgtcc tttcctaata aaatgaggaa attgcatcgc
     6841 attgtctgag taggtgtcat tctattctgg ggggtggggt ggggcaggac agcaaggggg
     6901 aggattggga agacaatagc aggcatgctg gggatgcggt gggctctatg gcttctgagg
     6961 cggaaagaac cagctggggc tctagggggt atccccacgc gccctgtagc ggcgcattaa
     7021 gcgcggcggg tgtggtggtt acgcgcagcg tgaccgctac acttgccagc gccctagcgc
     7081 ccgctccttt cgctttcttc ccttcctttc tcgccacgtt cgccggcttt ccccgtcaag
     7141 ctctaaatcg gggcatccct ttagggttcc gatttagtgc tttacggcac ctcgacccca
     7201 aaaaacttga ttagggtgat ggttcacgta gtgggccatc gccctgatag acggtttttc
     7261 gccctttgac gttggagtcc acgttcttta atagtggact cttgttccaa actggaacaa
     7321 cactcaaccc tatctcggtc tattcttttg atttataagg gattttgggg atttcggcct
     7381 attggttaaa aaatgagctg atttaacaaa aatttaacgc gaattaattc tgtggaatgt
     7441 gtgtcagtta gggtgtggaa agtccccagg ctccccaggc aggcagaagt atgcaaagca
     7501 tgcatctcaa ttagtcagca accaggtgtg gaaagtcccc aggctcccca gcaggcagaa
     7561 gtatgcaaag catgcatctc aattagtcag caaccatagt cccgccccta actccgccca
     7621 tcccgcccct aactccgccc agttccgccc attctccgcc ccatggctga ctaatttttt
     7681 ttatttatgc agaggccgag gccgcctctg cctctgagct attccagaag tagtgaggag
     7741 gcttttttgg aggcctaggc ttttgcaaaa agctcccggg agcttgtata tccattttcg
     7801 gatctgatca gcacgtgttg acaattaatc atcggcatag tatatcggca tagtataata
     7861 cgacaaggtg aggaactaaa ccatggccaa gttgaccagt gccgttccgg tgctcaccgc
     7921 gcgcgacgtc gccggagcgg tcgagttctg gaccgaccgg ctcgggttct cccgggactt
     7981 cgtggaggac gacttcgccg gtgtggtccg ggacgacgtg accctgttca tcagcgcggt
     8041 ccaggaccag gtggtgccgg acaacaccct ggcctgggtg tgggtgcgcg gcctggacga
     8101 gctgtacgcc gagtggtcgg aggtcgtgtc cacgaacttc cgggacgcct ccgggccggc
     8161 catgaccgag atcggcgagc agccgtgggg gcgggagttc gccctgcgcg acccggccgg
     8221 caactgcgtg cacttcgtgg ccgaggagca ggactgacac gtgctacgag atttcgattc
     8281 caccgccgcc ttctatgaaa ggttgggctt cggaatcgtt ttccgggacg ccggctggat
     8341 gatcctccag cgcggggatc tcatgctgga gttcttcgcc caccccaact tgtttattgc
     8401 agcttataat ggttacaaat aaagcaatag catcacaaat ttcacaaata aagcattttt
     8461 ttcactgcat tctagttgtg gtttgtccaa actcatcaat gtatcttatc atgtctgtat
     8521 accgtcgacc tctagctaga gcttggcgta atcatggtca tagctgtttc ctgtgtgaaa
     8581 ttgttatccg ctcacaattc cacacaacat acgagccgga agcataaagt gtaaagcctg
     8641 gggtgcctaa tgagtgagct aactcacatt aattgcgttg cgctcactgc ccgctttcca
     8701 gtcgggaaac ctgtcgtgcc agctgcatta atgaatcggc caacgcgcgg ggagaggcgg
     8761 tttgcgtatt gggcgctctt ccgcttcctc gctcactgac tcgctgcgct cggtcgttcg
     8821 gctgcggcga gcggtatcag ctcactcaaa ggcggtaata cggttatcca cagaatcagg
     8881 ggataacgca ggaaagaaca tgtgagcaaa aggccagcaa aaggccagga accgtaaaaa
     8941 ggccgcgttg ctggcgtttt tccataggct ccgcccccct gacgagcatc acaaaaatcg
     9001 acgctcaagt cagaggtggc gaaacccgac aggactataa agataccagg cgtttccccc
     9061 tggaagctcc ctcgtgcgct ctcctgttcc gaccctgccg cttaccggat acctgtccgc
     9121 ctttctccct tcgggaagcg tggcgctttc tcaatgctca cgctgtaggt atctcagttc
     9181 ggtgtaggtc gttcgctcca agctgggctg tgtgcacgaa ccccccgttc agcccgaccg
     9241 ctgcgcctta tccggtaact atcgtcttga gtccaacccg gtaagacacg acttatcgcc
     9301 actggcagca gccactggta acaggattag cagagcgagg tatgtaggcg gtgctacaga
     9361 gttcttgaag tggtggccta actacggcta cactagaagg acagtatttg gtatctgcgc
     9421 tctgctgaag ccagttacct tcggaaaaag agttggtagc tcttgatccg gcaaacaaac
     9481 caccgctggt agcggtggtt tttttgtttg caagcagcag attacgcgca gaaaaaaagg
     9541 atctcaagaa gatcctttga tcttttctac ggggtctgac gctcagtgga acgaaaactc
     9601 acgttaaggg attttggtca tgagattatc aaaaaggatc ttcacctaga tccttttaaa
     9661 ttaaaaatga agttttaaat caatctaaag tatatatgag taaacttggt ctgacagtta
     9721 ccaatgctta atcagtgagg cacctatctc agcgatctgt ctatttcgtt catccatagt
     9781 tgcctgactc cccgtcgtgt agataactac gatacgggag ggcttaccat ctggccccag
     9841 tgctgcaatg ataccgcgag acccacgctc accggctcca gatttatcag caataaacca
     9901 gccagccgga agggccgagc gcagaagtgg tcctgcaact ttatccgcct ccatccagtc
     9961 tattaattgt tgccgggaag ctagagtaag tagttcgcca gttaatagtt tgcgcaacgt
    10021 tgttgccatt gctacaggca tcgtggtgtc acgctcgtcg tttggtatgg cttcattcag
    10081 ctccggttcc caacgatcaa ggcgagttac atgatccccc atgttgtgca aaaaagcggt
    10141 tagctccttc ggtcctccga tcgttgtcag aagtaagttg gccgcagtgt tatcactcat
    10201 ggttatggca gcactgcata attctcttac tgtcatgcca tccgtaagat gcttttctgt
    10261 gactggtgag tactcaacca agtcattctg agaatagtgt atgcggcgac cgagttgctc
    10321 ttgcccggcg tcaatacggg ataataccgc gccacatagc agaactttaa aagtgctcat
    10381 cattggaaaa cgttcttcgg ggcgaaaact ctcaaggatc ttaccgctgt tgagatccag
    10441 ttcgatgtaa cccactcgtg cacccaactg atcttcagca tcttttactt tcaccagcgt
    10501 ttctgggtga gcaaaaacag gaaggcaaaa tgccgcaaaa aagggaataa gggcgacacg
    10561 gaaatgttga atactcatac tcttcctttt tcaatattat tgaagcattt atcagggtta
    10621 ttgtctcatg agcggataca tatttgaatg tatttagaaa aataaacaaa taggggttcc
    10681 gcgcacattt ccccgaaaag tgccacctga cgtc
//
