LOCUS       sequence-72818-e         745 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Retroviral Destination vector containing AttR1-AttR4 site. Use with 
            4-way LR reaction with cDNA in entry vector, marker in pBEG R2-L3 
            plasmids and shRNA in pBEG R3-L4 plasmid (module 2,3,and 4) .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 745)
  AUTHORS   Geiling B, Vandal G, Posner AR, de Bruyns A, Dutchak KL, Garnett S, 
            Dankort D
  TITLE     A modular lentiviral and retroviral construction system to rapidly 
            generate vectors for gene expression and gene knockdown in vitro and
            in vivo.
  JOURNAL   PLoS One. 2013 Oct 11;8(10):e76279. doi: 
            10.1371/journal.pone.0076279.
  PUBMED    24146852
REFERENCE   2  (bases 1 to 745)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2013-10-11"; volume: "8(10):e76279. doi"; pages: " 
            10.1371/journal.pone.0076279"
COMMENT     Sequence Label: pREG(R1-R4)   #BGV059
FEATURES             Location/Qualifiers
     source          1..745
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    38..162
                     /gene="mutant version of attR"
                     /label=attR1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     promoter        join(187..192,193..210,211..217)
                     /label=lac UV5 promoter
                     /note="E. coli lac promoter with an ""up"" mutation"
     primer_bind     complement(337..356)
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
ORIGIN
        1 tggaacggac ctgcaggaat tgatccgcgg ccatcaaaca agtttgtaca aaaaagctga
       61 acgagaaacg taaaatgata taaatatcaa tatattaaat tagattttgc ataaaaaaca
      121 gactacataa tactgtaaaa cacaacatat ccagtcacta tggcggccgc attaggcacc
      181 ccaggcttta cactttatgc ttccggctcg tataatgtgt ggattttgag ttaggatccg
      241 tcgagatttt caggagctaa ggaagctaaa atggagaaaa aaatcactgg atataccacc
      301 gttgatatat cccaatggca tcgtaaagaa cattttgagg catttcagtc agttgctcaa
      361 tgtacctata accagaccgt tcagctggat attacggcct ttttaaagac cgtaaagaaa
      421 aataagcaca agttttatcc ggcctttatt cacattcttg cccgcctgat gaatgctcat
      481 ccggaattcc gtatggcaat gaaagacggt gagctggtga tatgggatag tgttcaccct
      541 tgttacaccg ttttccatga gcaaactgaa acgttttcat cgctctggag tgaataccac
      601 gacgatttcc ggcagtttct acacatatat tcgcaagatg tggcgtgtta cggtgaaaac
      661 ctggcctatt tccctaaagg gtttattgag aatatgtttt tcgtctcagc caatccctgg
      721 gtgagtttca ccagttttga tttaa
//
