LOCUS       sequence-72826-e         963 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  For inserting selection gene between AttR2 and AttL3 sites for use 
            in Gateway recombination cloning system (corresponds to a module 3 
            plasmid).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 963)
  AUTHORS   Geiling B, Vandal G, Posner AR, de Bruyns A, Dutchak KL, Garnett S, 
            Dankort D
  TITLE     A modular lentiviral and retroviral construction system to rapidly 
            generate vectors for gene expression and gene knockdown in vitro and
            in vivo.
  JOURNAL   PLoS One. 2013 Oct 11;8(10):e76279. doi: 
            10.1371/journal.pone.0076279.
  PUBMED    24146852
REFERENCE   2  (bases 1 to 963)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2013-10-11"; volume: "8(10):e76279. doi"; pages: " 
            10.1371/journal.pone.0076279"
COMMENT     Sequence Label: pBEG R2-L3    #BGV193
FEATURES             Location/Qualifiers
     source          1..963
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    complement(23..147)
                     /gene="mutant version of attR"
                     /label=attR2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     protein_bind    complement(224..319)
                     /gene="mutant version of attL"
                     /label=attL3
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(348..367)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(349..367)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(374..391)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(374..390)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(383..405)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
ORIGIN
        1 cgccactagc agatctgccg gccatagtga ctggatatgt tgtgttttac agtattatgt
       61 agtctgtttt ttatgcaaaa tctaatttaa tatattgata tttatatcat tttacgtttc
      121 tcgttcagct ttcttgtaca aagtggtgct agcgcggccg cacgcgtgct gaggagacat
      181 ctagactttc cctcagcgtc gacgatatcg gcgcgccccc gggcaacttt attatacaaa
      241 gttggcatta taaaaaagca ttgctcatca atttgttgca acgaacaggt cactatcagt
      301 caaaataaaa tcattattta ctagtgttaa cttcgatccc caattcgccc tatagtgagt
      361 cgtattacaa ttcactggcc gtcgttttac aacgtcgtga ctgggaaaac cctggcgtta
      421 cccaacttaa tcgccttgca gcacatcccc ctttcgccag ctggcgtaat agcgaagagg
      481 cccgcaccga tcgcccttcc caacagttgc gcagcctgaa tggcgaatga gcttgcgccg
      541 tcccgtcaag tcagcgtaat gctctgccag tgttacaacc aattaaccaa ttctgattag
      601 aaaaactcat cgagcatcaa atgaaactgc aatttattca tatcaggatt atcaatacca
      661 tatttttgaa aaagccgttt ctgtaatgaa ggagaaaact caccgaggca gttccatagg
      721 atggcaagat cctggtatcg gtctgcgatt ccgactcgtc caacatcaat acaacctatt
      781 aatttcccct cgtcaaaaat aaggttatca agtgagaaat caccatgagt gacgactgaa
      841 tccggtgaga atggcaaaag tttatgcatt tctttccaga cttgttcaac aggccagcca
      901 ttacgctcgt catcaaaatc actcgcatca accaaaccgt tattcattcg tgattgcgcc
      961 tga
//
