LOCUS       sequence-7283-ex       11090 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11090)
  AUTHORS   Marr RA, Guan H, Rockenstein E, Kindy M, Gage FH, Verma I, Masliah 
            E, Hersh LB
  TITLE     Neprilysin regulates amyloid Beta peptide levels.
  JOURNAL   J Mol Neurosci. 2004  . 22(1-2):5-11.
  PUBMED    14742905
REFERENCE   2  (bases 1 to 11090)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Mol 
            Neurosci. 2004  . 22(1-2):5-11."
COMMENT     Sequence Label: pCSC-SP-PW-Nep (aka: pBOB-NEP)
FEATURES             Location/Qualifiers
     source          1..11090
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(44..63)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        235..614
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        616..814
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     LTR             832..1012
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    1056..1181
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1674..1907
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             2092..2136
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2388..2505
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     enhancer        2528..2831
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        2832..3035
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     3032..3056
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     misc_feature    5896..6484
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(5949..5969)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(6367..6378)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             6891..7071
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(7097..7114)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    7103..7327
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      7373..7801
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(7460..7479)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     7670..7691
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(7810..7830)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        7815..8145
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      7996..8131
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     8058..8077
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     promoter        8193..8240
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             8259..8633
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     polyA_signal    8763..8884
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(8800..8819)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     8854..8873
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(8933..8949)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(8933..8949)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(8946..8968)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    8957..8973
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(8981..8987,8988..9005,9006..9011))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    9026..9047
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(9164..9181)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(9335..9923)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(9415..9434)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(10094..10885,10886..10954))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     10717..10736
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(10955..11059)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 gacggatcgg gagatctccc gatcccctat ggtcgactct cagtacaatc tgctctgatg
       61 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg
      121 cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc
      181 ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgttgacatt
      241 gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata
      301 tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc
      361 cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc
      421 attgacgtca atgggtggac tatttacggt aaactgccca cttggcagta catcaagtgt
      481 atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt
      541 atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca
      601 tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg
      661 actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc
      721 aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg
      781 gtaggcgtgt acggtgggag gtctatataa gcagcgcgtt ttgcctgtac tgggtctctc
      841 tggttagacc agatctgagc ctgggagctc tctggctaac tagggaaccc actgcttaag
      901 cctcaataaa gcttgccttg agtgcttcaa gtagtgtgtg cccgtctgtt gtgtgactct
      961 ggtaactaga gatccctcag acccttttag tcagtgtgga aaatctctag cagtggcgcc
     1021 cgaacaggga cctgaaagcg aaagggaaac cagagctctc tcgacgcagg actcggcttg
     1081 ctgaagcgcg cacggcaaga ggcgaggggc ggcgactggt gagtacgcca aaaattttga
     1141 ctagcggagg ctagaaggag agagatgggt gcgagagcgt cagtattaag cgggggagaa
     1201 ttagatcgcg atgggaaaaa attcggttaa ggccaggggg aaagaaaaaa tataaattaa
     1261 aacatatagt atgggcaagc agggagctag aacgattcgc agttaatcct ggcctgttag
     1321 aaacatcaga aggctgtaga caaatactgg gacagctaca accatccctt cagacaggat
     1381 cagaagaact tagatcatta tataatacag tagcaaccct ctattgtgtg catcaaagga
     1441 tagagataaa agacaccaag gaagctttag acaagataga ggaagagcaa aacaaaagta
     1501 agaccaccgc acagcaagcg gccgctgatc ttcagacttg gaggaggaga tatgagggac
     1561 aattggagaa gtgaattata taaatataaa gtagtaaaaa ttgaaccatt aggagtagca
     1621 cccaccaagg caaagagaag agtggtgcag agagaaaaaa gagcagtggg aataggagct
     1681 ttgttccttg ggttcttggg agcagcagga agcactatgg gcgcagcctc aatgacgctg
     1741 acggtacagg ccagacaatt attgtctggt atagtgcagc agcagaacaa tttgctgagg
     1801 gctattgagg cgcaacagca tctgttgcaa ctcacagtct ggggcatcaa gcagctccaa
     1861 gcaagaatcc tagctgtgga aagataccta aaggatcaac agctcctagg gatttggggt
     1921 tgctctggaa aactcatttg caccactgct gtgccttgga atgctagttg gagtaataaa
     1981 tctctggaac agatctggaa tcacacgacc tggatggagt gggacagaga aattaacaat
     2041 tacacaagct taatacactc cttaattgaa gaatcgcaaa accagcaaga aaagaatgaa
     2101 caagaattat tggaattaga taaatgggca agtttgtgga attggtttaa cataacaaat
     2161 tggctgtggt atataaaatt attcataatg atagtaggag gcttggtagg tttaagaata
     2221 gtttttgctg tactttctat agtgaataga gttaggcagg gatattcacc attatcgttt
     2281 cagacccacc tcccaatccc gaggggaccc gacaggcccg aaggaataga agaagaaggt
     2341 ggagagagag acagagacag atccattcga ttagtgaacg gatcaacttt taaaagaaaa
     2401 ggggggattg gggggtacag tgcaggggaa agaatagtag acataatagc aacagacata
     2461 caaactaaag aattacaaaa acaaattaca aaaattcaaa attttatcga taagcttggg
     2521 agttccgcgt tacataactt acggtaaatg gcccgcctgg ctgaccgccc aacgaccccc
     2581 gcccattgac gtcaataatg acgtatgttc ccatagtaac gccaataggg actttccatt
     2641 gacgtcaatg ggtggagtat ttacggtaaa ctgcccactt ggcagtacat caagtgtatc
     2701 atatgccaag tacgccccct attgacgtca atgacggtaa atggcccgcc tggcattatg
     2761 cccagtacat gaccttatgg gactttccta cttggcagta catctacgta ttagtcatcg
     2821 ctattaccat ggtgatgcgg ttttggcagt acatcaatgg gcgtggatag cggtttgact
     2881 cacggggatt tccaagtctc caccccattg acgtcaatgg gagtttgttt tggcaccaaa
     2941 atcaacggga ctttccaaaa tgtcgtaaca actccgcccc attgacgcaa atgggcggta
     3001 ggcgtgtacg gtgggaggtc tatataagca gagctcgttt agtgaaccgt cagatcgcct
     3061 ggagacgcca tccacgctgt tttgacctcc atagaagaca ccgactctag aggatccacc
     3121 ggtggccgcc tgggcccgtt cgcgtcgcgc ccaggaccct gcggccgtgg aggaggcgtg
     3181 ccctgggaag gagccgctac tgggacctga agaattttag gtgatgggca agtcagaaag
     3241 tcagatggat ataactgata tcaacactcc aaagccaaag aagaaacagc gatggactcg
     3301 actggagatc agcctctcgg tccttgtcct gctcctcacc atcatagctg tgagaatgat
     3361 cgcactctat gcaacctacg atgatggtat ttgcaagtca tcagactgca taaaatcagc
     3421 tgctcgactg atccaaaaca tggatgccac cactgagcct tgtagagact ttttcaaata
     3481 tgcttgcgga ggctggttga aacgtaatgt cattcccgag accagctccc gttacggcaa
     3541 ctttgacatt ttaagagatg aactagaagt cgttttgaaa gatgtccttc aagaacccaa
     3601 aactgaagat atagtagcag tgcagaaagc aaaagcattg tacaggtctt gtataaatga
     3661 atctgctatt gatagcagag gtggagaacc tctactcaaa ctgttaccag acatatatgg
     3721 gtggccagta gcaacagaaa actgggagca aaaatatggt gcttcttgga cagctgaaaa
     3781 agctattgca caactgaatt ctaaatatgg gaaaaaagtc cttattaatt tgtttgttgg
     3841 cactgatgat aagaattctg tgaatcatgt aattcatatt gaccaacctc gacttggcct
     3901 cccttctaga gattactatg aatgcactgg aatctataaa gaggcttgta cagcatatgt
     3961 ggattttatg atttctgtgg ccagattgat tcgtcaggaa gaaagattgc ccatcgatga
     4021 aaaccagctt gctttggaaa tgaataaagt tatggaattg gaaaaagaaa ttgccaatgc
     4081 tacggctaaa cctgaagatc gaaatgatcc aatgcttctg tataacaaga tgagattggc
     4141 ccagatccaa aataactttt cactagagat caatgggaag ccattcagct ggttgaattt
     4201 cacaaatgaa atcatgtcaa ctgtgaatat tagtattaca aatgaggaag atgtggttgt
     4261 ttatgctcca gaatatttaa ccaaacttaa gcccattctt accaaatatt ctgccagaga
     4321 tcttcaaaat ttaatgtcct ggagattcat aatggatctt gtaagcagcc tcagccgaac
     4381 ctacaaggag tccagaaatg ctttccgcaa ggccctttat ggtacaacct cagaaacagc
     4441 aacttggaga cgttgtgcaa actatgtcaa tgggaatatg gaaaatgctg tggggaggct
     4501 ttatgtggaa gcagcatttg ctggagagag taaacatgtg gtcgaggatt tgattgcaca
     4561 gatccgagaa gtttttattc agactttaga tgacctcact tggatggatg ccgagacaaa
     4621 aaagagagct gaagaaaagg ccttagcaat taaagaaagg atcggctatc ctgatgacat
     4681 tgtttcaaat gataacaaac tgaataatga gtacctcgag ttgaactaca aagaagatga
     4741 atacttcgag aacataattc aaaatttgaa attcagccaa agtaaacaac tgaagaagct
     4801 ccgagaaaag gtggacaaag atgagtggat aagtggagca gctgtagtca atgcatttta
     4861 ctcttcagga agaaatcaga tagtcttccc agccggcatt ctgcagcccc ccttctttag
     4921 tgcccagcag tccaactcat tgaactatgg gggcatcggc atggtcatag gacacgaaat
     4981 cacccatggc ttcgatgaca atggcagaaa ctttaacaaa gatggagacc tcgttgactg
     5041 gtggactcaa cagtctgcaa gtaactttaa ggagcaatcc cagtgcatgg tgtatcagta
     5101 tggaaacttt tcctgggacc tggcaggtgg acagcacctt aatggaatta atacactggg
     5161 agaaaacatt gctgataatg gaggtcttgg tcaagcatac agagcctatc agaattatat
     5221 taaaaagaat ggcgaagaaa aattacttcc tggacttgac ctaaatcaca aacaactatt
     5281 tttcttgaac tttgcacagg tgtggtgtgg aacctatagg ccagagtatg cggttaactc
     5341 cattaaaaca gatgtgcaca gtccaggcaa tttcaggatt attgggactt tgcagaactc
     5401 tgcagagttt tcagaagcct ttcactgccg caagaattca tacatgaatc cagaaaagaa
     5461 gtgccgggtt tggtgatctt caaaagaagc attgcagccc ttggctagac ttgccaacac
     5521 cacagaaatg gggaattctc taatcgaaag aaaatgggcc ctaggggtca ctgtactgac
     5581 ttgagggtga ttaacagaga gggcaccatc acaatacaga taacattagg ttgtcctaga
     5641 aagggtgtgg agggaggaag ggggtctaag gtctatcaag tcaatcattt ctcactgtgt
     5701 acataatgct taatttctaa agataatatt actgtttatt tctgtttctc atatggtcta
     5761 cgcgaacgtc tcgagtttaa acctggaaaa acatggagca atcacaagta gcaacacagc
     5821 agctaccaat gctgcttgtg cctggctaga agcacaagag gaggaggagg tgggttttcc
     5881 agtcacacct cagacaatca acctctggat tacaaaattt gtgaaagatt gactggtatt
     5941 cttaactatg ttgctccttt tacgctatgt ggatacgctg ctttaatgcc tttgtatcat
     6001 gctattgctt cccgtatggc tttcattttc tcctccttgt ataaatcctg gttgctgtct
     6061 ctttatgagg agttgtggcc cgttgtcagg caacgtggcg tggtgtgcac tgtgtttgct
     6121 gacgcaaccc ccactggttg gggcattgcc accacctgtc agctcctttc cgggactttc
     6181 gctttccccc tccctattgc cacggcggaa ctcatcgccg cctgccttgc ccgctgctgg
     6241 acaggggctc ggctgttggg cactgacaat tccgtggtgt tgtcggggaa gctgacgtcc
     6301 tttccatggc tgctcgcctg tgttgccacc tggattctgc gcgggacgtc cttctgctac
     6361 gtcccttcgg ccctcaatcc agcggacctt ccttcccgcg gcctgctgcc ggctctgcgg
     6421 cctcttccgc gtcttcgcct tcgccctcag acgagtcgga tctccctttg ggccgcctcc
     6481 ccgcctggaa ttcgagctcg gtacctttaa gaccaatgac ttacaaggca gctgtagatc
     6541 ttagccactt tttaaaagaa aaggggggac tggaagggct aattcactcc caaagaagac
     6601 aagatatcct tgatctgtgg atctaccaca cacaaggcta cttccctgat tgacagaact
     6661 acacaccagg gccaggggtc agatatccac tgacctttgg atggtgctac aagctagtac
     6721 cagttgagcc agataagata gaagaggcca ataaaggaga gaacaccagc ttgttacacc
     6781 ctgtgagcct gcatgggatg gatgacccgg agagagaagt gttagagtgg aggtttgaca
     6841 gccgcctagc atttcatcac gtggcccgag agctgcatcc ggactgtact gggtctctct
     6901 ggttagacca gatctgagcc tgggagctct ctggctaact agggaaccca ctgcttaagc
     6961 ctcaataaag cttgccttga gtgcttcaag tagtgtgtgc ccgtctgttg tgtgactctg
     7021 gtaactagag atccctcaga cccttttagt cagtgtggaa aatctctagc agggccgctt
     7081 taaacccgct gatcagcctc gactgtgcct tctagttgcc agccatctgt tgtttgcccc
     7141 tcccccgtgc cttccttgac cctggaaggt gccactccca ctgtcctttc ctaataaaat
     7201 gaggaaattg catcgcattg tctgagtagg tgtcattcta ttctgggggg tggggtgggg
     7261 caggacagca agggggagga ttgggaagac aatagcaggc atgctgggga tgcggtgggc
     7321 tctatggctt ctgaggcgga aagaaccagc tggggctcta gggggtatcc ccacgcgccc
     7381 tgtagcggcg cattaagcgc ggcgggtgtg gtggttacgc gcagcgtgac cgctacactt
     7441 gccagcgccc tagcgcccgc tcctttcgct ttcttccctt cctttctcgc cacgttcgcc
     7501 ggctttcccc gtcaagctct aaatcggggc atccctttag ggttccgatt tagtgcttta
     7561 cggcacctcg accccaaaaa acttgattag ggtgatggtt cacgtagtgg gccatcgccc
     7621 tgatagacgg tttttcgccc tttgacgttg gagtccacgt tctttaatag tggactcttg
     7681 ttccaaactg gaacaacact caaccctatc tcggtctatt cttttgattt ataagggatt
     7741 ttggggattt cggcctattg gttaaaaaat gagctgattt aacaaaaatt taacgcgaat
     7801 taattctgtg gaatgtgtgt cagttagggt gtggaaagtc cccaggctcc ccaggcaggc
     7861 agaagtatgc aaagcatgca tctcaattag tcagcaacca ggtgtggaaa gtccccaggc
     7921 tccccagcag gcagaagtat gcaaagcatg catctcaatt agtcagcaac catagtcccg
     7981 cccctaactc cgcccatccc gcccctaact ccgcccagtt ccgcccattc tccgccccat
     8041 ggctgactaa ttttttttat ttatgcagag gccgaggccg cctctgcctc tgagctattc
     8101 cagaagtagt gaggaggctt ttttggaggc ctaggctttt gcaaaaagct cccgggagct
     8161 tgtatatcca ttttcggatc tgatcagcac gtgttgacaa ttaatcatcg gcatagtata
     8221 tcggcatagt ataatacgac aaggtgagga actaaaccat ggccaagttg accagtgccg
     8281 ttccggtgct caccgcgcgc gacgtcgccg gagcggtcga gttctggacc gaccggctcg
     8341 ggttctcccg ggacttcgtg gaggacgact tcgccggtgt ggtccgggac gacgtgaccc
     8401 tgttcatcag cgcggtccag gaccaggtgg tgccggacaa caccctggcc tgggtgtggg
     8461 tgcgcggcct ggacgagctg tacgccgagt ggtcggaggt cgtgtccacg aacttccggg
     8521 acgcctccgg gccggccatg accgagatcg gcgagcagcc gtgggggcgg gagttcgccc
     8581 tgcgcgaccc ggccggcaac tgcgtgcact tcgtggccga ggagcaggac tgacacgtgc
     8641 tacgagattt cgattccacc gccgccttct atgaaaggtt gggcttcgga atcgttttcc
     8701 gggacgccgg ctggatgatc ctccagcgcg gggatctcat gctggagttc ttcgcccacc
     8761 ccaacttgtt tattgcagct tataatggtt acaaataaag caatagcatc acaaatttca
     8821 caaataaagc atttttttca ctgcattcta gttgtggttt gtccaaactc atcaatgtat
     8881 cttatcatgt ctgtataccg tcgacctcta gctagagctt ggcgtaatca tggtcatagc
     8941 tgtttcctgt gtgaaattgt tatccgctca caattccaca caacatacga gccggaagca
     9001 taaagtgtaa agcctggggt gcctaatgag tgagctaact cacattaatt gcgttgcgct
     9061 cactgcccgc tttccagtcg ggaaacctgt cgtgccagct gcattaatga atcggccaac
     9121 gcgcggggag aggcggtttg cgtattgggc gctcttccgc ttcctcgctc actgactcgc
     9181 tgcgctcggt cgttcggctg cggcgagcgg tatcagctca ctcaaaggcg gtaatacggt
     9241 tatccacaga atcaggggat aacgcaggaa agaacatgtg agcaaaaggc cagcaaaagg
     9301 ccaggaaccg taaaaaggcc gcgttgctgg cgtttttcca taggctccgc ccccctgacg
     9361 agcatcacaa aaatcgacgc tcaagtcaga ggtggcgaaa cccgacagga ctataaagat
     9421 accaggcgtt tccccctgga agctccctcg tgcgctctcc tgttccgacc ctgccgctta
     9481 ccggatacct gtccgccttt ctcccttcgg gaagcgtggc gctttctcaa tgctcacgct
     9541 gtaggtatct cagttcggtg taggtcgttc gctccaagct gggctgtgtg cacgaacccc
     9601 ccgttcagcc cgaccgctgc gccttatccg gtaactatcg tcttgagtcc aacccggtaa
     9661 gacacgactt atcgccactg gcagcagcca ctggtaacag gattagcaga gcgaggtatg
     9721 taggcggtgc tacagagttc ttgaagtggt ggcctaacta cggctacact agaaggacag
     9781 tatttggtat ctgcgctctg ctgaagccag ttaccttcgg aaaaagagtt ggtagctctt
     9841 gatccggcaa acaaaccacc gctggtagcg gtggtttttt tgtttgcaag cagcagatta
     9901 cgcgcagaaa aaaaggatct caagaagatc ctttgatctt ttctacgggg tctgacgctc
     9961 agtggaacga aaactcacgt taagggattt tggtcatgag attatcaaaa aggatcttca
    10021 cctagatcct tttaaattaa aaatgaagtt ttaaatcaat ctaaagtata tatgagtaaa
    10081 cttggtctga cagttaccaa tgcttaatca gtgaggcacc tatctcagcg atctgtctat
    10141 ttcgttcatc catagttgcc tgactccccg tcgtgtagat aactacgata cgggagggct
    10201 taccatctgg ccccagtgct gcaatgatac cgcgagaccc acgctcaccg gctccagatt
    10261 tatcagcaat aaaccagcca gccggaaggg ccgagcgcag aagtggtcct gcaactttat
    10321 ccgcctccat ccagtctatt aattgttgcc gggaagctag agtaagtagt tcgccagtta
    10381 atagtttgcg caacgttgtt gccattgcta caggcatcgt ggtgtcacgc tcgtcgtttg
    10441 gtatggcttc attcagctcc ggttcccaac gatcaaggcg agttacatga tcccccatgt
    10501 tgtgcaaaaa agcggttagc tccttcggtc ctccgatcgt tgtcagaagt aagttggccg
    10561 cagtgttatc actcatggtt atggcagcac tgcataattc tcttactgtc atgccatccg
    10621 taagatgctt ttctgtgact ggtgagtact caaccaagtc attctgagaa tagtgtatgc
    10681 ggcgaccgag ttgctcttgc ccggcgtcaa tacgggataa taccgcgcca catagcagaa
    10741 ctttaaaagt gctcatcatt ggaaaacgtt cttcggggcg aaaactctca aggatcttac
    10801 cgctgttgag atccagttcg atgtaaccca ctcgtgcacc caactgatct tcagcatctt
    10861 ttactttcac cagcgtttct gggtgagcaa aaacaggaag gcaaaatgcc gcaaaaaagg
    10921 gaataagggc gacacggaaa tgttgaatac tcatactctt cctttttcaa tattattgaa
    10981 gcatttatca gggttattgt ctcatgagcg gatacatatt tgaatgtatt tagaaaaata
    11041 aacaaatagg ggttccgcgc acatttcccc gaaaagtgcc acctgacgtc
//
