LOCUS       sequence-72843-e         951 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses human codon optimized Cas9 D10A mutant which functions as 
            a nickase for genome engineering.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 951)
  AUTHORS   Mali P, Yang L, Esvelt KM, Aach J, Guell M, Dicarlo JE, Norville JE,
            Church GM
  TITLE     RNA-Guided Human Genome Engineering via Cas9.
  JOURNAL   Science. 2013 Feb 15;339(6121):823-6. doi: 10.1126/science.1232033
  PUBMED    23287722
REFERENCE   2  (bases 1 to 951)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1126/science"; 
            journalName: "Science"; date: "2013-02-15- 15"; volume: "339"; 
            issue: "6121"; pages: "823-6"
COMMENT     Sequence Label: hCas9_D10A
FEATURES             Location/Qualifiers
     source          1..951
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(92..103)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
ORIGIN
        1 aacttccgtt gtgtgatcag tttggcgttc agcagctgcc gccaataatt tttcattttc
       61 ttgacaactt cttctgaggg gacgttatca ctcttccctc tatttttatc ggatcttgtc
      121 aacactttat tatcaataga atcatctttg agaaaagact ggggcacgat atgatccacg
      181 tcgtagtcgg agagccgatt gatgtccagt tcctgatcca cgtacatgtc cctgccgttc
      241 tgcaggtagt acaggtagag cttctcattc tgaagctggg tgttttcaac tgggtgttcc
      301 ttaaggattt gggaccccag ttcttttata ccctcttcaa tcctcttcat cctttcccta
      361 ctgttcttct gtcccttctg ggtagtttgg ttctctcggg ccatctcgat aacgatattc
      421 tcgggcttat gccttcccat tactttgacg agttcatcca cgaccttaac ggtctgcagt
      481 attccctttt tgatagctgg gctacctgca agattagcga tgtgctcgtg aagactgtcc
      541 ccctggccag aaacttgtgc tttctggatg tcctccttaa aggtgagaga gtcatcatgg
      601 atcaactgca tgaagttcct gttggcaaat ccatcggact taagaaaatc caggattgtc
      661 tttccactct gcttgtctcg gatcccattg atcagttttc ttgacagccg cccccatcct
      721 gtatatcggc gcctcttgag ctgtttcatg actttgtcgt cgaagagatg agcgtaagtt
      781 ttcaagcgtt cttcaatcat ctccctatct tcaaacaacg taagggtgag gacaatgtcc
      841 tcaagaatgt cctcgttctc ctcattgtcc aggaagtcct tgtctttaat gattttcagg
      901 agatcgtgat acgttcccag ggatgcgttg aagcgatcct ccactccgct g
//
