LOCUS       sequence-72844-e         930 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Supplies the selection marker (blasticidin resistance) and IRES with
            flanked by AttR2 and AttL3 sites for Gateway cloning recombination 
            reaction. (This corresponds to a module 3 plasmid)..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 930)
  AUTHORS   Geiling B, Vandal G, Posner AR, de Bruyns A, Dutchak KL, Garnett S, 
            Dankort D
  TITLE     A modular lentiviral and retroviral construction system to rapidly 
            generate vectors for gene expression and gene knockdown in vitro and
            in vivo.
  JOURNAL   PLoS One. 2013 Oct 11;8(10):e76279. doi: 
            10.1371/journal.pone.0076279.
  PUBMED    24146852
REFERENCE   2  (bases 1 to 930)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2013-10-11"; volume: "8(10):e76279. doi"; pages: " 
            10.1371/journal.pone.0076279"
COMMENT     Sequence Label: pBEG R2-iBlast-L3   #BGV204
FEATURES             Location/Qualifiers
     source          1..930
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    32..127
                     /gene="mutant version of attL"
                     /label=attL3
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(759..778)
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
ORIGIN
        1 gggcgaattg gggatcgaag ttaacactag taaataatga ttttattttg actgatagtg
       61 acctgttcgt tgcaacaaat tgatgagcaa tgctttttta taatgccaac tttgtataat
      121 aaagttgccc gggggcgcgc cgatatctcg agacgcgtga tccttaattt cgggtatatt
      181 tgagtggaat gagttcttca atcgtagttt tgactaactt gccattcatt tctattaaca
      241 caaaacaatc tggtgcatag tctgaaatca actccctaca cataccacaa ggacttacca
      301 ctcgaatact tctatctact tcgtcagaat aagggtgtct aacagctaca atcgtgtcaa
      361 aatccttttg tccattcgaa actgcactac caatcgcaat ggcttctgca caaacagtta
      421 ctcgtcctat atacgcttca atatgtactg ccgaaatgat ttctcctgtt ttcgtacgaa
      481 ttgccgctcc cacatgatgt ttattatcct cataaagcat tgtaatcttc tctgtcgcta
      541 cttctactaa ttctagatcc tgttgagaaa tgttaaatgt tttcatggtg gatcaagctt
      601 atcatcgtgt ttttcaaagg aaaaccacgt ccccgtggtt cggggggcct agacgttttt
      661 ttaacctcga ctaaacacat gtaaagcatg tgcaccgagg ccccagatca gatcccatac
      721 aatggggtac cttctgggca tccttcagcc ccttgttgaa tacgcttgag gagagccatt
      781 tgactctttc cacaactatc caactcacaa cgtggcactg gggttgtgcc gcctttgcag
      841 gtgtatctta tacacgtggc ttttggccgc agaggcacct gtcgccaggt ggggggttcc
      901 gctgcctgca aagggtcgct acagacgttg
//
