LOCUS       sequence-72847-e         953 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses human codon optimized Cas9 D10A mutant which functions as 
            a nickase for genome engineering.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 953)
  AUTHORS   Mali P, Yang L, Esvelt KM, Aach J, Guell M, Dicarlo JE, Norville JE,
            Church GM
  TITLE     RNA-Guided Human Genome Engineering via Cas9.
  JOURNAL   Science. 2013 Feb 15;339(6121):823-6. doi: 10.1126/science.1232033
  PUBMED    23287722
REFERENCE   2  (bases 1 to 953)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1126/science"; 
            journalName: "Science"; date: "2013-02-15- 15"; volume: "339"; 
            issue: "6121"; pages: "823-6"
COMMENT     Sequence Label: hCas9_D10A
FEATURES             Location/Qualifiers
     source          1..953
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      108..117
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 gtttagtgaa ccgtcagatc gcctggagac gccatccacg ctgttttgac ctccatagaa
       61 gacaccggga ccgatccagc ctccggactc tagaggatcg aacccttgcc accatggaca
      121 agaagtactc cattgggctc gctatcggca caaacagcgt cggctgggcc gtcattacgg
      181 acgagtacaa ggtgccgagc aaaaaattca aagttctggg caataccgat cgccacagca
      241 taaagaagaa cctcattggc gccctcctgt tcgactccgg ggagacggcc gaagccacgc
      301 ggctcaaaag aacagcacgg cgcagatata cccgcagaaa gaatcggatc tgctacctgc
      361 aggagatctt tagtaatgag atggctaagg tggatgactc tttcttccat aggctggagg
      421 agtccttttt ggtggaggag gataaaaagc acgagcgcca cccaatcttt ggcaatatcg
      481 tggacgaggt ggcgtaccat gaaaagtacc caaccatata tcatctgagg aagaagcttg
      541 tagacagtac tgataaggct gacttgcggt tgatctatct cgcgctggcg catatgatca
      601 aatttcgggg acacttcctc atcgaggggg acctgaaccc agacaacagc gatgtcgaca
      661 aactctttat ccaactggtt cagacttaca atcagctttt cgaagagaac ccgatcaacg
      721 catccggagt tgacgccaaa gcaatcctga gcgctaggct gtccaaatcc cggcggctcg
      781 aaaacctcat cgcacagctc cctggggaga agaagaacgg cctgtttggt aatcttatcg
      841 ccctgtcact cgggctgacc cccaacttta aatctaactt cgacctggcc gaagatgcca
      901 agcttcaact gagcaaagac acctacgatg atgatctcga caatctgctg gcc
//
