LOCUS       sequence-72850-e         905 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses human codon optimized Cas9 D10A mutant which functions as 
            a nickase for genome engineering.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 905)
  AUTHORS   Mali P, Yang L, Esvelt KM, Aach J, Guell M, Dicarlo JE, Norville JE,
            Church GM
  TITLE     RNA-Guided Human Genome Engineering via Cas9.
  JOURNAL   Science. 2013 Feb 15;339(6121):823-6. doi: 10.1126/science.1232033
  PUBMED    23287722
REFERENCE   2  (bases 1 to 905)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1126/science"; 
            journalName: "Science"; date: "2013-02-15- 15"; volume: "339"; 
            issue: "6121"; pages: "823-6"
COMMENT     Sequence Label: hCas9_D10A
FEATURES             Location/Qualifiers
     source          1..905
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(99..110)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
ORIGIN
        1 attatcgaac ttccgttgtg tgatcagttt ggcgttcagc agctgccgcc aataattttt
       61 cattttcttg acaacttctt ctgaggggac gttatcactc ttccctctat ttttatcgga
      121 tcttgtcaac actttattat caatagaatc atctttgaga aaagactggg gcacgatatg
      181 atccacgtcg tagtcggaga gccgattgat gtccagttcc tgatccacgt acatgtccct
      241 gccgttctgc aggtagtaca ggtagagctt ctcattctga agctgggtgt tttcaactgg
      301 gtgttcctta aggatttggg accccagttc ttttataccc tcttcaatcc tcttcatcct
      361 ttccctactg ttcttctgtc ccttctgggt agtttggttc tctcgggcca tctcgataac
      421 gatattctcg ggcttatgcc ttcccattac tttgacgagt tcatccacga ccttaacggt
      481 ctgcagtatt ccctttttga tagctgggct acctgcaaga ttagcgatgt gctcgtgaag
      541 actgtccccc tggccagaaa cttgtgcttt ctggatgtcc tccttaaagg tgagagagtc
      601 atcatggatc aactgcatga agttcctgtt ggcaaatcca tcggacttaa gaaaatccag
      661 gattgtcttt ccactctgct tgtctcggat cccattgatc agttttcttg acagccgccc
      721 ccatcctgta tatcggcgcc tcttgagctg tttcatgact ttgtcgtcga agagatgagc
      781 gtaagttttc aagcgttctt caatcatctc cctatcttca aacaacgtaa gggtgaggac
      841 aatgtcctca agaatgtcct cgttctcctc attgtccagg aagtccttgt ctttaatgat
      901 tttca
//
