LOCUS       sequence-72854-e         964 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Contains the Gateway selection cassette (ccdB ChlorR) flanked by 
            AttR1 and AttR4 sites for the creation of Destination vectors .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 964)
  AUTHORS   Geiling B, Vandal G, Posner AR, de Bruyns A, Dutchak KL, Garnett S, 
            Dankort D
  TITLE     A modular lentiviral and retroviral construction system to rapidly 
            generate vectors for gene expression and gene knockdown in vitro and
            in vivo.
  JOURNAL   PLoS One. 2013 Oct 11;8(10):e76279. doi: 
            10.1371/journal.pone.0076279.
  PUBMED    24146852
REFERENCE   2  (bases 1 to 964)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2013-10-11"; volume: "8(10):e76279. doi"; pages: " 
            10.1371/journal.pone.0076279"
COMMENT     Sequence Label: pBEG R1-ChlorR-R4   #BGV049
FEATURES             Location/Qualifiers
     source          1..964
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    17..141
                     /gene="mutant version of attR"
                     /label=attR1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     promoter        join(172..177,178..195,196..202)
                     /label=lac UV5 promoter
                     /note="E. coli lac promoter with an ""up"" mutation"
     CDS             256..936
                     /codon_start=1
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQAGRNLE
                     DPAY"
     primer_bind     complement(322..341)
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
ORIGIN
        1 tagcagatct gctagcacaa gtttgtacaa aaaagctgaa cgagaaacgt aaaatgatat
       61 aaatatcaat atattaaatt agattttgca taaaaaacag actacataat actgtaaaac
      121 acaacatatc cagtcactat ggccggcgcg gccgcattag gcaccccagg ctttacactt
      181 tatgcttccg gctcgtataa tgtgtggatt ttgagttagg atccgtcgag attttcagga
      241 gctaaggaag ctaaaatgga gaaaaaaatc actggatata ccaccgttga tatatcccaa
      301 tggcatcgta aagaacattt tgaggcattt cagtcagttg ctcaatgtac ctataaccag
      361 accgttcagc tggatattac ggccttttta aagaccgtaa agaaaaataa gcacaagttt
      421 tatccggcct ttattcacat tcttgcccgc ctgatgaatg ctcatccgga attccgtatg
      481 gcaatgaaag acggtgagct ggtgatatgg gatagtgttc acccttgtta caccgttttc
      541 catgagcaaa ctgaaacgtt ttcatcgctc tggagtgaat accacgacga tttccggcag
      601 tttctacaca tatattcgca agatgtggcg tgttacggtg aaaacctggc ctatttccct
      661 aaagggttta ttgagaatat gtttttcgtc tcagccaatc cctgggtgag tttcaccagt
      721 tttgatttaa acgtggccaa tatggacaac ttcttcgccc ccgttttcac catgggcaaa
      781 tattatacgc aaggcgacaa ggtgctgatg ccgctggcga ttcaggttca tcatgccgtt
      841 tgtgatggct tccatgtcgg cagaatgctt aatgaattac aacagtactg cgatgagtgg
      901 caggcggggc gtaatctaga ggatccggct tactaaaagc cagataacag tatgcgtatt
      961 ttgc
//
