LOCUS       sequence-72857-e         992 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  To create an AttR3-AttL4 flanked Entry vector encoding a 
            miR30-embedded shRNA to knockdown targeted gene expression. (This 
            corresponds to a module 4 plasmid)..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 992)
  AUTHORS   Geiling B, Vandal G, Posner AR, de Bruyns A, Dutchak KL, Garnett S, 
            Dankort D
  TITLE     A modular lentiviral and retroviral construction system to rapidly 
            generate vectors for gene expression and gene knockdown in vitro and
            in vivo.
  JOURNAL   PLoS One. 2013 Oct 11;8(10):e76279. doi: 
            10.1371/journal.pone.0076279.
  PUBMED    24146852
REFERENCE   2  (bases 1 to 992)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2013-10-11"; volume: "8(10):e76279. doi"; pages: " 
            10.1371/journal.pone.0076279"
COMMENT     Sequence Label: pBEG R3-miRNA(ccdB)-L4   #BGV228
FEATURES             Location/Qualifiers
     source          1..992
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    complement(54..177)
                     /gene="mutant version of attR"
                     /label=attR3
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     ncRNA           223..316
                     /label=5' miR-30a
                     /note="sequence upstream of the 71-nt precursor of the 
                     human miR-30a microRNA (Zeng et al., 2002)"
                     /ncRNA_class="miRNA"
     promoter        join(337..342,343..360,361..367)
                     /label=lac UV5 promoter
                     /note="E. coli lac promoter with an ""up"" mutation"
     primer_bind     complement(487..506)
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
ORIGIN
        1 gcagatctgc tagactttcc ctcagcggat ctgctagtgc ggccgcgccg gcccatagtg
       61 actggatatg ttgtgtttta cagtattatg tagtctgttt tttatgcaaa atctaattta
      121 atatattgat atttatatca ttttacgttt ctcgttcaac tttattatac atagttggct
      181 agtggatcca gcgctggcgc gccatttaaa tcgatagttt gtttgaatga ggcttcagta
      241 ctttacagaa tcgttgcctg cacatcttgg aaacacttgc tgggattact tcttcaggtt
      301 aacccaacag aaggctcgag attaggcacc ccaggcttta cactttatgc ttccggctcg
      361 tataatgtgt ggattttgag ttaggatccg tcgagatttt caggagctaa ggaagctaaa
      421 atggagaaaa aaatcactgg atataccacc gttgatatat cccaatggca tcgtaaagaa
      481 cattttgagg catttcagtc agttgctcaa tgtacctata accagaccgt tcagctggat
      541 attacggcct ttttaaagac cgtaaagaaa aataagcaca agttttatcc ggcctttatt
      601 cacattcttg cccgcctgat gaatgctcat ccggagttcc gtatggcaat gaaagacggt
      661 gagctggtga tatgggatag tgttcaccct tgttacaccg ttttccatga gcaaactgaa
      721 acgttttcat cgctctggag tgaataccac gacgatttcc ggcagtttct acacatatat
      781 tcgcaagatg tggcgtgtta cggtgaaaac ctggcctatt tccctaaagg gtttattgag
      841 aatatgtttt tcgtctcagc caatccctgg gtgagtttca ccagttttga tttaaacgtg
      901 gccaatatgg acaacttctt cgcccccgtt ttcaccatgg gcaaatatta tacgcaaggc
      961 gacaaggtgc tgatgccgct ggcgattcag gt
//
