LOCUS       sequence-72858-e        1070 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Shuttle vector E. coli/B.meg.; encoding the RNA polymerase of the 
            phage SP6 under control of the xylose inducible promoter PxylA.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1070)
  AUTHORS   Stammen S, Schuller F, Dietrich S, Gamer M, Biedendieck R, Jahn D
  TITLE     Application of Escherichia coli phage K1E DNA-dependent RNA 
            polymerase for in vitro RNA synthesis and in vivo protein production
            in Bacillus megaterium.
  JOURNAL   Appl Microbiol Biotechnol. 2010 Sep;88(2):529-39. doi: 
            10.1007/s00253-010-2732-y. Epub 2010 Jul 2.
  PUBMED    20596705
REFERENCE   2  (bases 1 to 1070)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1007/s00253-010-2732-y"; journalName: "Appl Microbiol 
            Biotechnol"; date: "2010-09"; volume: "88"; issue: "2"; pages: 
            "529-39"
COMMENT     Sequence Label: pSP6-RNAP
FEATURES             Location/Qualifiers
     source          1..1070
                     /mol_type="genomic DNA"
                     /organism="unspecified"
ORIGIN
        1 tctaatatta atttttgatt catttttttt actaaagctt gatctgcaat ttgaataata
       61 accactcctt tgtttatcca ccgaactaag ttggtgtttt ttgaagcttg aattagatat
      121 ttaaaagtat catatctaat attataacta aattttctaa aaaaaacatt gaaataaaca
      181 tttattttgt atatgatgag ataaagttag tttattggat aaacaaacta actcaattaa
      241 gatagttgat ggataaactt gttcacttaa atcaaagggg gaaatgacaa atggtccaaa
      301 ctagttcgaa gatctccgga gctcaagatt tacacgctat ccagcttcaa ttagaagaag
      361 agatgtttaa tggtggcatt cgtcgcttcg aagcagatca acaacgccag attgcagcag
      421 gtagcgagag cgacacagca tggaaccgcc gcctgttgtc agaacttatt gcacctatgg
      481 ctgaaggcat tcaggcttat aaagaagagt acgaaggtaa gaaaggtcgt gcacctcgcg
      541 cattggcttt cttacaatgt gtagaaaatg aagttgcagc atacatcact atgaaagttg
      601 ttatggatat gctgaatacg gatgctaccc ttcaggctat tgcaatgagt gtagcagaac
      661 gcattgaaga ccaagtgcgc ttttctaagc tagaaggtca cgccgctaaa tactttgaga
      721 aggttaagaa gtcactcaag gctagccgta ctaagtcata tcgtcacgct cataacgtag
      781 ctgtagttgc tgaaaaatca gttgcagaaa aggacgcgga ctttgaccgt tgggaggcgt
      841 ggccaaaaga aactcaattg cagattggta ctaccttgct tgaaatctta gaaggtagcg
      901 ttttctataa tggtgaacct gtatttatgc gtgctatgcg cacttatggc ggaaagacta
      961 tttactactt acaaacttct gaaagtgtag gccagtggat tagcgcattc aaagagcacg
     1021 tagcgcaatt aagcccagct tatgcccctt gcgtaatccc tcctcgtcct
//
