LOCUS       sequence-72860-e        1013 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Shuttle vector E. coli/B.meg.; encoding the RNA polymerase of the 
            phage K1E under control of the xylose inducible promoter PxylA.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1013)
  AUTHORS   Stammen S, Schuller F, Dietrich S, Gamer M, Biedendieck R, Jahn D
  TITLE     Application of Escherichia coli phage K1E DNA-dependent RNA 
            polymerase for in vitro RNA synthesis and in vivo protein production
            in Bacillus megaterium.
  JOURNAL   Appl Microbiol Biotechnol. 2010 Sep;88(2):529-39. doi: 
            10.1007/s00253-010-2732-y. Epub 2010 Jul 2.
  PUBMED    20596705
REFERENCE   2  (bases 1 to 1013)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1007/s00253-010-2732-y"; journalName: "Appl Microbiol 
            Biotechnol"; date: "2010-09"; volume: "88"; issue: "2"; pages: 
            "529-39"
COMMENT     Sequence Label: pK1E-RNAP
FEATURES             Location/Qualifiers
     source          1..1013
                     /mol_type="genomic DNA"
                     /organism="unspecified"
ORIGIN
        1 atattaattt ttgattcatt ttttttacta aagcttgatc tgcaatttga ataataacca
       61 ctcctttgtt tatccaccga actaagttgg tgttttttga agcttgaatt agatatttaa
      121 aagtatcata tctaatatta taactaaatt ttctaaaaaa aacattgaaa taaacattta
      181 ttttgtatat gatgagataa agttagttta ttggataaac aaactaactc aattaagata
      241 gttgatggat aaacttgttc acttaaatca aagggggaaa tgacaaatgg tccaaactag
      301 tcaagaccta cacgctattc aacttcaact tgaagaagaa atgtttaacg gcggcatccg
      361 tcgctttgaa gcggaccaac aacgccagat tgcatccggt aatgaatcag acacggcatg
      421 gaatcgccgc ttattgtccg aactaatcgc acctatggcg gaaggtattc aggcttacaa
      481 ggaagaatac gaaggcaaga gaggtcgtgc accacgtgca ttagctttca ttaactgcgt
      541 aggaaacgaa gtggcagcat atatcaccat gaaaatcgtt atggatatgc tgaatacaga
      601 cgttacctta caagctatcg ctatgaatgt agcagaccgc atcgaggacc aagtacgttt
      661 tagcaagctg gaaggtcacg cagctaagta ctttgagaaa gtgaagaagt cgcttaaggc
      721 tagcaagact aaatcttatc gtcatgcgca caacgtagcg gtagtagctg agaaatctgt
      781 tgctgaccgt gacgcggatt tctcccgttg ggaggcatgg cctaaagaca ccttgctgca
      841 aatcggtatg accttgctcg aaatattgga gaatagtgta ttcttcaatg gtcaacctgt
      901 cttccttcgt accttgcgca ctaacggcgg aaagcatggt gtttactatt tacagaccag
      961 tgaacatgtt ggcgagtgga taactgcatt taaggaacat gtagcgcagc tca
//
