LOCUS       sequence-72864-e         911 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Shuttle vector E. coli/B.meg.; gfp-expression under control of 
            RNAP-SP6-inducible promoter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 911)
  AUTHORS   Stammen S, Schuller F, Dietrich S, Gamer M, Biedendieck R, Jahn D
  TITLE     Application of Escherichia coli phage K1E DNA-dependent RNA 
            polymerase for in vitro RNA synthesis and in vivo protein production
            in Bacillus megaterium.
  JOURNAL   Appl Microbiol Biotechnol. 2010 Sep;88(2):529-39. doi: 
            10.1007/s00253-010-2732-y. Epub 2010 Jul 2.
  PUBMED    20596705
REFERENCE   2  (bases 1 to 911)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1007/s00253-010-2732-y"; journalName: "Appl Microbiol 
            Biotechnol"; date: "2010-09"; volume: "88"; issue: "2"; pages: 
            "529-39"
COMMENT     Sequence Label: pPSP6+t-gfp
FEATURES             Location/Qualifiers
     source          1..911
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     8..26
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
ORIGIN
        1 ctataaaaat aggcgtatca cgaggccctt tcgtcttcaa gaattcctgt tataaaaaaa
       61 ggatcaattt tgaactctct cccaaagttg atcccttaac gatttagaaa tccctttgag
      121 aatgtttata tacattcaag gtaaccagcc aactaatgac aatgattcct gaaaaaagta
      181 ataacaaatt actatacaga taagttgact gatcaacttc cataggtaac aacctttgat
      241 caagtaaggg tatggataat aaaccaccta caattgcaat acctgttccc tctgataaaa
      301 agctggtaaa gttaagcaaa ctcattccag caccagcttc ctgctgtttc aagctacttg
      361 aaacaattgt tgatataact gttttggtga acgaaagccc acctaaaaca aatacgatta
      421 taattgtcat gaaccatgat gttgtttcta aaagaaagga agcagttaaa aagctaacag
      481 aaagaaatgt aactccgatg tttaacacgt ataaaggacc tcttctatca acaagtatcc
      541 caccaatgta gccgaaaata atgacactca ttgttccagg gaaaataatt acacttccga
      601 tttcggcagt acttagctgg tgaacatctt tcatcatata aggaaccata gagacaaacc
      661 ctgctactgt tccaaatata attcccccac aaagaactcc aatcataaaa ggtatatttt
      721 tccctaatcc gggatcaaca aaaggatctg ttactttcct gatatgtttt acaaatatca
      781 ggaatgacag cacgctaacg ataagaaaag aaatgctata tgatgttgta aacaacataa
      841 aaaatacaat gcctacagac attagtataa ttcctttgat atcaaaatga ccttttatcc
      901 ttacttcttt c
//
