LOCUS       sequence-72865-e         781 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Recombinant protein production.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 781)
  AUTHORS   Posner M, Kiss AJ, Skiba J, Drossman A, Dolinska MB, Hejtmancik JF, 
            Sergeev YV
  TITLE     Functional validation of hydrophobic adaptation to physiological 
            temperature in the small heat shock protein alphaA-crystallin.
  JOURNAL   PLoS One. 2012;7(3):e34438. doi: 10.1371/journal.pone.0034438. Epub 
            2012 Mar 29.
  PUBMED    22479631
REFERENCE   2  (bases 1 to 781)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2012"; volume: "7(3):e34438. doi"; pages: " 
            10.1371/journal.pone.0034438. Epub 2012 Mar 29"
COMMENT     Sequence Label: Danio rerio cryaa V62T
FEATURES             Location/Qualifiers
     source          1..781
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             590..607
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(660..678)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      674..721
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
ORIGIN
        1 ctttaagaag gagatataca tatggatatt gcgatccaac acccttggtt cagacgcaca
       61 ctgggctacc ccacccgact ctttgatcag ttctttggag aaggcctgtt cgattatgac
      121 ctattcccct tcaccacttc aactgtcagc ccttactatc gacactcact cttccgcaac
      181 atcctggact cctccaactc gggtacctct gaggtgaggt ctgacagaga aaaatttaca
      241 gtttatctgg atgtgaaaca tttctctcct gatgagctca gtgtcaaggt gacagatgac
      301 tatgtggaga tccagggcaa gcatggagaa agacaggatg atcatggcta catctcccgt
      361 gagttccatc gtcgctaccg cctgccttcc aatgtggacc agtctgccat cacctgcaca
      421 ctgtctgctg atggcctgct cactctttgt ggacccaaga caagtggcat agatgctggc
      481 cgtggagatc gcaccatccc tgttacccgc gaggacaaga gcaactcagg ctcttcctcc
      541 tagggatccg aattcgagct ccgtcgacaa gcttgcggcc gcactcgagc accaccacca
      601 ccaccactga gatccggctg ctaacaaagc ccgaaaggaa gctgagttgg ctgctgccac
      661 cgctgagcaa taactagcat aaccccttgg ggcctctaaa cgggtcttga ggggtttttt
      721 gctgaaagga ggaactatat ccggattggc gaatgggacg cgccctgtag cggcgcatta
      781 a
//
