LOCUS       sequence-72867-e         766 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Recombinant protein production .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 766)
  AUTHORS   Posner M, Kiss AJ, Skiba J, Drossman A, Dolinska MB, Hejtmancik JF, 
            Sergeev YV
  TITLE     Functional validation of hydrophobic adaptation to physiological 
            temperature in the small heat shock protein alphaA-crystallin.
  JOURNAL   PLoS One. 2012;7(3):e34438. doi: 10.1371/journal.pone.0034438. Epub 
            2012 Mar 29.
  PUBMED    22479631
REFERENCE   2  (bases 1 to 766)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2012"; volume: "7(3):e34438. doi"; pages: " 
            10.1371/journal.pone.0034438. Epub 2012 Mar 29"
COMMENT     Sequence Label: Oncorhynchus kisutch cryaa
FEATURES             Location/Qualifiers
     source          1..766
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(15..32)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     RBS             580..602
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     primer_bind     complement(633..652)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(634..652)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
ORIGIN
        1 gcagccggat ctcagtggtg gtggtggtgg tgctcgagtt aagaggaggg ggaggcgtta
       61 gtcttgtcgt cgcgggtgac ggggatgctg cggtcgccac ggccgaggtc gccaccgcca
      121 ccgctgacct ttgggccaca aagggtcagg aggccgtcgg tggacagtgt gcaggagata
      181 gccgattggt ccacgctgga ggggaggcgg tagcggcggt ggaactcacg ggagatgtaa
      241 ccgtggtcgt cctgtctctc tccatgcttt ccctggatct ccacatagtc atcagtcact
      301 tttacgttga gttcatcagg agagaagtgc ttcacatcca tgaagactga gaacttgtcc
      361 ctgtcggacc tcacctcaga catgccagag ttggtggagt ccaggaagtt gcggaacagc
      421 gactgtctgt agtaggggct gatggtggag gcagcgtagg ggaacaggtc atagtcgaac
      481 atgccctccc cgaaaaactg gtcaaagaga cgagcgggat acatggagcc catggcacgt
      541 ctgaaccagg ggtgctggat ggcaatatcc atatgtatat ctccttctta aagttaaaca
      601 aaattatttc tagagggaaa ccgttgtggt ctccctatag tgagtcgtat taatttcgcg
      661 ggatcgagat ctcgggcagc gttgggtcct ggccacgggt gcgcatgatc gtgctcctgt
      721 cgttgaggac ccggctaggc tggcggggtt gccttactgg ttagca
//
