LOCUS       sequence-72869-e        1043 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Shuttle vector E. coli/B.meg.; gfp-expression under control of 
            RNAP-K1E-inducible promoter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1043)
  AUTHORS   Stammen S, Schuller F, Dietrich S, Gamer M, Biedendieck R, Jahn D
  TITLE     Application of Escherichia coli phage K1E DNA-dependent RNA 
            polymerase for in vitro RNA synthesis and in vivo protein production
            in Bacillus megaterium.
  JOURNAL   Appl Microbiol Biotechnol. 2010 Sep;88(2):529-39. doi: 
            10.1007/s00253-010-2732-y. Epub 2010 Jul 2.
  PUBMED    20596705
REFERENCE   2  (bases 1 to 1043)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1007/s00253-010-2732-y"; journalName: "Appl Microbiol 
            Biotechnol"; date: "2010-09"; volume: "88"; issue: "2"; pages: 
            "529-39"
COMMENT     Sequence Label: pPK1E-2+t-gfp
FEATURES             Location/Qualifiers
     source          1..1043
                     /mol_type="genomic DNA"
                     /organism="unspecified"
ORIGIN
        1 aaaggatcaa ttttgaactc tctcccaaag ttgatccctt aacgatttag aaatcccttt
       61 gagaatgttt atatacattc aaggtaacca gccaactaat gacaatgatt cctgaaaaaa
      121 gtaataacaa attactatac agataagttg actgatcaac ttccataggt aacaaccttt
      181 gatcaagtaa gggtatggat aataaaccac ctacaattgc aatacctgtt ccctctgata
      241 aaaagctggt aaagttaagc aaactcattc cagcaccagc ttcctgctgt ttcaagctac
      301 ttgaaacaat tgttgatata actgttttgg tgaacgaaag cccacctaaa acaaatacga
      361 ttataattgt catgaaccat gatgttgttt ctaaaagaaa ggaagcagtt aaaaagctaa
      421 cagaaagaaa tgtaactccg atgtttaaca cgtataaagg acctcttcta tcaacaagta
      481 tcccaccaat gtagccgaaa ataatgacac tcattgttcc agggaaaata attacacttc
      541 cgatttcggc agtacttagc tggtgaacat ctttcatcat ataaggaacc atagagacaa
      601 accctgctac tgttccaaat ataattcccc cacaaagaac tccaatcata aaaggtatat
      661 ttttccctaa tccgggatca acaaaaggat ctgttacttt cctgatatgt tttacaaata
      721 tcaggaatga cagcacgcta acgataagaa aagaaatgct atatgatgtt gtaaacaaca
      781 taaaaaatac aatgcctaca gacattagta taattccttt gatatcaaaa tgacctttta
      841 tccttacttc tttctttaat aatttcataa gaaacggaac agtgataatt gttatcatag
      901 gaatgagtag aagataggac caatgaatat aatgggctat cattccacca atcgctggac
      961 cgactccttc tcccatggct actatcgatc caataagacc aaatgcttta cccctatttt
     1021 cctttggaat atagcgcgca act
//
