LOCUS       sequence-72873-e         917 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 917)
  AUTHORS   Deuschle K, Okumoto S, Fehr M, Looger LL, Kozhukh L, Frommer WB
  TITLE     Construction and optimization of a family of genetically encoded 
            metabolite sensors by semirational protein engineering.
  JOURNAL   Protein Sci. 2005 Sep . 14(9):2304-14.
  PUBMED    16131659
REFERENCE   2  (bases 1 to 917)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Protein 
            Sci. 2005 Sep . 14(9):2304-14."
COMMENT     Sequence Label: pRSET FLIPglu-170nDelta13
FEATURES             Location/Qualifiers
     source          1..917
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(546..567)
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     895..914
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
ORIGIN
        1 tgtaactgtt cagttttgat gcctttatcg ttcaattctt taatcacgta agtggtacgt
       61 gcttctgcat ccggatggcc cggttcacct ttcagcagta cgaactgaat ctgaccgtct
      121 ttgttcagat cccaaccctg attcgccgcc cagtgtttag caatcaaatc gccttgaata
      181 atgccggact ctttggagtc agtgccaacg tagtaggctt tgtcgtagct atccagcgcc
      241 ttacgagacg gttctttgtt gaagaaaacc accggcacgt tttgcccacg cgctttctca
      301 atcaccgtac ccgcagctgc cgggtcaacc aggttgattg ccagtgcctt cacccctttc
      361 gccagcaata cgtcgatctg atcgttctgc ttggactggt cattctgaga atcattcatc
      421 agcagctgaa catctggcgc ggctttcgca tcttgctcaa tagccttgcg cactacagac
      481 ataaagttat cgtcgtactt atagattgtt acaccaatgc gagtatcagc gatcccggcg
      541 gcggtcacga actccagcag gaccatgtga tcgcgcttct cgttggggtc tttgctcagg
      601 gcggactggg tgctcaggta gtggttgtcg ggcagcagca cggggccgtc gccgatgggg
      661 gtgttctgct ggtagtggtc ggcgagctgc acgctgccgt cctcgatgtt gtggcggatc
      721 ttgaagttgg ccttgatgcc gttcttctgc ttgtcggcgg tgatatagac gttgtggctg
      781 atgtagttgt actccagctt gtgccccagg atgttgccgt cctccttgaa gtcgatgccc
      841 ttcagctcga tgcggttcac cagggtgtcg ccctcgaact tcacctcggc gcgggtcttg
      901 tagttgccgt cgtcctt
//
