LOCUS       sequence-7288-ex        8288 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8288)
  AUTHORS   Sivakumar S, Porter-Goff M, Patel PK, Benoit K, Rhind N
  TITLE     In vivo labeling of fission yeast DNA with thymidine and thymidine 
            analogs.
  JOURNAL   Methods. 2004 Jul . 33(3):213-9.
  PUBMED    15157888
REFERENCE   2  (bases 1 to 8288)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Methods. 
            2004 Jul . 33(3):213-9."
COMMENT     Sequence Label: pFS119
FEATURES             Location/Qualifiers
     source          1..8288
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        38..142
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(143..211,212..1003)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERSPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(361..380)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      complement(1045..1554)
                     /direction=LEFT
                     /label=M13 ori
                     /note="M13 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(1240..1261)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     1448..1467
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     rep_origin      1665..2253
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     2154..2173
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     protein_bind    2314..2335
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(2350..2355,2356..2373,2374..2380)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    2388..2404
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     2393..2415
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     2412..2428
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     2412..2428
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     CDS             complement(5303..6433)
                     /codon_start=1
                     /product="herpes simplex virus thymidine kinase"
                     /label=HSV TK
                     /note="confers sensitivity to nucleoside analogs such as 
                     acyclovir or ganciclovir"
                     /translation="MASYPGHQHASAFDQAARSRGHSNRRTALRPRRQQEATEVRPEQK
                     MPTLLRVYIDGPHGMGKTTTTQLLVALGSRDDIVYVPEPMTYWRVLGASETIANIYTTQ
                     HRLDQGEISAGDAAVVMTSAQITMGMPYAVTDAVLAPHIGGEAGSSHAPPPALTLIFDR
                     HPIAALLCYPAARYLMGSMTPQAVLAFVALIPPTLPGTNIVLGALPEDRHIDRLAKRQR
                     PGERLDLAMLAAIRRVYGLLANTVRYLQGGGSWREDWGQLSGTAVPPQGAEPQSNAGPR
                     PHIGDTLFTLFRAPELLAPNGDLYNVFAWALDVLAKRLRPMHVFILDYDQSPAGCRDAL
                     LQLTSGMIQTHVTTPGSIPTICDLARTFAREMGEAN"
     rep_origin      7253..8034
                     /label=ars1
                     /note="Schizosaccharomyces pombe autonomously replicating 
                     sequence ars1"
     primer_bind     complement(8089..8106)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(8089..8105)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(8098..8120)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
ORIGIN
        1 gttaactacg tcaggtggca cttttcgggg aaatgtgcgc ggaaccccta tttgtttatt
       61 tttctaaata cattcaaata tgtatccgct catgagacaa taaccctgat aaatgcttca
      121 ataatattga aaaaggaaga gtatgagtat tcaacatttc cgtgtcgccc ttattccctt
      181 ttttgcggca ttttgccttc ctgtttttgc tcacccagaa acgctggtga aagtaaaaga
      241 tgctgaagat cagttgggtg cacgagtggg ttacatcgaa ctggatctca acagcggtaa
      301 gatccttgag agttttcgcc ccgaagaacg ttctccaatg atgagcactt ttaaagttct
      361 gctatgtggc gcggtattat cccgtgttga cgccgggcaa gagcaactcg gtcgccgcat
      421 acactattct cagaatgact tggttgagta ctcaccagtc acagaaaagc atcttacgga
      481 tggcatgaca gtaagagaat tatgcagtgc tgccataacc atgagtgata acactgcggc
      541 caacttactt ctgacaacga tcggaggacc gaaggagcta accgcttttt tgcacaacat
      601 gggggatcat gtaactcgcc ttgatcgttg ggaaccggag ctgaatgaag ccataccaaa
      661 cgacgagcgt gacaccacga tgcctgtagc aatggcaaca acgttgcgca aactattaac
      721 tggcgaacta cttactctag cttcccggca acaattaata gactggatgg aggcggataa
      781 agttgcagga ccacttctgc gctcggccct tccggctggc tggtttattg ctgataaatc
      841 tggagccggt gagcgtgggt ctcgcggtat cattgcagca ctggggccag atggtaagcc
      901 ctcccgtatc gtagttatct acacgacggg gagtcaggca actatggatg aacgaaatag
      961 acagatcgct gagataggtg cctcactgat taagcattgg taactgtcag accaagttta
     1021 ctcatatata ctttagattg atttaccccg gttgataatc agaaaagccc caaaaacagg
     1081 aagattgtat aagcaaatat ttaaattgta aacgttaata ttttgttaaa attcgcgtta
     1141 aatttttgtt aaatcagctc attttttaac caataggccg aaatcggcaa aatcccttat
     1201 aaatcaaaag aatagcccga gatagggttg agtgttgttc cagtttggaa caagagtcca
     1261 ctattaaaga acgtggactc caacgtcaaa gggcgaaaaa ccgtctatca gggcgatggc
     1321 ccactacgtg aaccatcacc caaatcaagt tttttggggt cgaggtgccg taaagcacta
     1381 aatcggaacc ctaaagggag cccccgattt agagcttgac ggggaaagcg aacgtggcga
     1441 gaaaggaagg gaagaaagcg aaaggagcgg gcgctagggc gctggcaagt gtagcggtca
     1501 cgctgcgcgt aaccaccaca cccgccgcgc ttaatgcgcc gctacagggc gcgtaaaagg
     1561 atctaggtga agatcctttt tgataatctc atgaccaaaa tcccttaacg tgagttttcg
     1621 ttccactgag cgtcagaccc cgtagaaaag atcaaaggat cttcttgaga tccttttttt
     1681 ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc taccagcggt ggtttgtttg
     1741 ccggatcaag agctaccaac tctttttccg aaggtaactg gcttcagcag agcgcagata
     1801 ccaaatactg ttcttctagt gtagccgtag ttaggccacc acttcaagaa ctctgtagca
     1861 ccgcctacat acctcgctct gctaatcctg ttaccagtgg ctgctgccag tggcgataag
     1921 tcgtgtctta ccgggttgga ctcaagacga tagttaccgg ataaggcgca gcggtcgggc
     1981 tgaacggggg gttcgtgcac acagcccagc ttggagcgaa cgacctacac cgaactgaga
     2041 tacctacagc gtgagctatg agaaagcgcc acgcttcccg aagggagaaa ggcggacagg
     2101 tatccggtaa gcggcagggt cggaacagga gagcgcacga gggagcttcc agggggaaac
     2161 gcctggtatc tttatagtcc tgtcgggttt cgccacctct gacttgagcg tcgatttttg
     2221 tgatgctcgt caggggggcg gagcctatgg aaaaacgcca gcaacgcggc ctttttacgg
     2281 ttcctggcct tttgctggcc ttttgctcac atgtaatgtg agttagctca ctcattaggc
     2341 accccaggct ttacacttta tgcttccggc tcgtatgttg tgtggaattg tgagcggata
     2401 acaatttcac acaggaaaca gctatgacca tgattacgcc aagctacgta atacgactca
     2461 ctagtcttaa tattgcattt tttgcattaa tatgcgttaa tttaattggg aacatggtca
     2521 acggggtttt atttgggttt cttactatta ttttttgatt gctttatagc atattatatt
     2581 gggttcttta attttctttc aactgcttca cagcacatta ttcaggattc ttattttttt
     2641 taattgcgtc gcagcacatt attcgggggg tttttatttt taatttttaa tcacttttta
     2701 gaatacattt tacaatctag aatttcaaaa taaaatatta tttaaaacaa aaagcaagca
     2761 aaatcattta acagttatgt ctatggtcgc ctatgcagaa taatttttcc aaccaacttt
     2821 ttctaattta tcagcctttt ctaaaacaat ctctttcata ttgtccataa agctctccat
     2881 agcagccaaa agggagggtt gaaatgtagc aagtatacga caggctaaaa taccggcatt
     2941 ttggctatta ttgatagcaa cagtggcgac agggacacct cgaggcatct gaacaataga
     3001 gtgaagagag tcaactccgt caagagtgct tccttttaca ggaacgccga ttactggaag
     3061 aggtgtcatt gcagcaacca taccaggcaa atgagcggca ccaccagcac cagcaataat
     3121 cacacgcaac cctcttgaag ctgcggtacg agcataagta accatgcgat ctggtgtgcg
     3181 gtgagctgaa acaatagtaa gttcgtaagg caccttgaat tcatctaaaa tgacggcagc
     3241 atctttcatc ttgcttaaat cagaatccga acccatgata ataccaacaa caggtgattc
     3301 gacaggatct ttgacgtcaa gaagcatctg atacctacgt tcacattctt gaggtgaatc
     3361 agaaatgatg gtaacgtgtc ccatcttgcg accctttcta gattcagctt taccataaag
     3421 atgcaaagtt gcaccgggaa tggacagaga acgtttagcg atctttgaaa cataatcagg
     3481 atcatcagta cctaaaatat ttaccatcaa cgcatgagtt gtggaagtcg agagttgggt
     3541 gttgatttcg ctgaaaggaa gaccacatat ggcccgtaag tgagcttcaa actgagaagt
     3601 tgggcaagct tcaatggtgt agtgacctga attgtgaggc cgaggagcaa tttcgttgag
     3661 taaaatggtc tcaccatctt gcaaaacaaa catctctaca ccatatatac cagcgccttc
     3721 aaaagtgcgc accgcatcca tggcaagggt ttgagcacgc tgttgaattg agaagggaag
     3781 acgagcaggg gcatatacta aatgacaaac attatccttt tgaatggtct cagttgtagg
     3841 ataagcataa acttttccgt ctaaactccg tactaccatc actgcaattt ccatggagaa
     3901 aggaacgaac ttttcaacat aaagcggacg atcaccaagt gctttgatgg cagtaggaat
     3961 ctcagatggt tgatgaacaa cgtaatttcc acgaccgtcg taagccaatg ttttactttt
     4021 cagtacaaaa ggataaccaa actcttggcc tgctttttca acactttcct ggtcaggtgc
     4081 atcgcaaaat tcaggaagtg caatcttaaa aacctgtaaa tgctgttttt gaagatattt
     4141 gtcttgaatg catcgcagag ttgcaggaga gggttcaaca gcaacagatt tcgtaacggc
     4201 tgccaaggca tcagtgttaa tatgctcaat ttcagttgtt aataacgtgc acttcttaga
     4261 cagttcaaca attgcatcgg ggtcagtaaa tgatgcatca atgtgctcac gtcctccatc
     4321 aatttgtttg gcaggagaat ttgctgcatc caagatgatg catttgatgt ttaagcgatg
     4381 ggctgcctct accatcattc ggcccaattg accacctcca aggatcccta caacctgttt
     4441 ttcgctcata ttcgatgaag tatgtatata ccttggcagc tcagcttaat gagatggtaa
     4501 atgttgaaaa aacgaatctt tttttcaaag ttgtttattg tagtgagtta gtgcggagtt
     4561 tattatttga gttttgcatc gtattaattc tgtcatcgtt acatttccaa gcattgtgaa
     4621 atgcaacttt aaagcagcat acgctaaaat caatatagct ttgctatttt ttcaagaaat
     4681 aaatgtatat gtcgttatcc atatataaag caacgtttgt tgtcgttata ccttatactg
     4741 caccaggctg gatttcgttt acctcaccac caagttgaaa gaatacttgt cgtcgaacca
     4801 agtgcagaat acggtctatt aaaagtcgtc catgtttcaa tgtgcgtatc actatgtccg
     4861 attggcggct tacagaaaat gtacttgacc aaaacatacc tgaaactaaa cccagggagt
     4921 ccaagactcg cctcgaggaa attcagcgat tagcattgtt tcaagaggaa ctcgacatta
     4981 tcgaagaatt ggatgacccc gtggatgttt ggtatcgctg catcgaatgg ctcttggaaa
     5041 caagattttt gggcatggaa acggtcaaca aaatgcttga tgatgctatt cagtacttag
     5101 aaagatgtcg cttcgcttaa tgatgttcgt catcttctca tacagcttgc gaaaattaaa
     5161 caatcatacg aaactcctga tgaattgcaa caggcggcca agcaatttta ccaattggca
     5221 tcgaaaggca tagggatctt cgaatgcatc gcgcgcaccg tacgtctcga ggaattcctg
     5281 caggatccgg ggatcccgtg tttcagttag cctcccccat ctcccgggca aacgtgcgcg
     5341 ccaggtcgca gatcgtcggt atggagcctg gggtggtgac gtgggtctgg atcatcccgg
     5401 aggtaagttg cagcagggcg tcccggcagc cggcgggcga ttggtcgtaa tccaggataa
     5461 agacgtgcat gggacggagg cgtttggcca agacgtccaa ggcccaggca aacacgttgt
     5521 acaggtcgcc gttgggggcc agcaactcgg gggcccgaaa cagggtaaat aacgtgtccc
     5581 cgatatgggg tcgtgggccc gcgttgctct ggggctcggc accctggggc ggcacggccg
     5641 tccccgaaag ctgtccccaa tcctcccgcc acgacccgcc gccctgcaga taccgcaccg
     5701 tattggcaag cagcccgtaa acgcggcgaa tcgcggccag catagccagg tcaagccgct
     5761 cgccggggcg ctggcgtttg gccaggcggt cgatgtgtct gtcctccgga agggccccca
     5821 acacgatgtt tgtgccgggc aaggtcggcg ggatgagggc cacgaacgcc agcacggcct
     5881 ggggggtcat gctgcccata aggtatcgcg cggccgggta gcacaggagg gcggcgatgg
     5941 gatggcggtc gaagatgagg gtgagggccg ggggcggggc atgtgaactc ccagcctccc
     6001 ccccgatatg aggagccaga acggcgtcgg tcacggcata aggcatgccc attgttatct
     6061 gggcgcttgt cattaccacc gccgcgtccc cggccgatat ctcaccctgg tcgaggcggt
     6121 gttgtgtggt gtagatgttc gcgattgtct cggaagcccc cagcacccgc cagtaagtca
     6181 tcggctcggg tacgtagacg atatcgtcgc gcgaacccag ggccaccagc agttgcgtgg
     6241 tggtggtttt ccccatcccg tggggaccgt ctatataaac ccgcagtagc gtgggcattt
     6301 tctgctccgg gcggacttcc gtggcttctt gctgccggcg agggcgcaac gccgtacgtc
     6361 ggttgctatg gccgcgagaa cgcgcagcct ggtcgaacgc agacgcgtgc tgatggccgg
     6421 ggtacgaagc catacgggat cctctagagt cgacctgcag caattctctt gcttaaagaa
     6481 aagaaaagcg aaggcacctg tccaccaccc actatttata ccatgggagg cgtcaaaccg
     6541 agtttttgcg aaaggattat cgcacctctc cccaagtaac atcggcaaag caaaaacgga
     6601 gcggaagacc aatgagacgc ggaaggggag cgccgaatga ggaaagcgag agagaaagaa
     6661 gtggatggaa tgattccccg ccaaagtgga cacatttcgg gaatcttcag gaagcatgga
     6721 tcaccttatg gacaagaaga taatccggaa gccaatagga gggcgacagg gggtcagcga
     6781 taagcgtcgg aaaaaacttt atgatatggt aaatacgtag gaggttacgg tgcagaggag
     6841 gggccggtag cgagtgatag cgagggaaag acgatcgcta tcgggccgta aggaggaaat
     6901 gggaggttgg gggaaggatg cgttggaatg cggagtagag aaattgaaga gagagagagg
     6961 acacacctag agagaaagaa atggatatag agaaagagaa aaaataagag gaatacggat
     7021 acgatggagt gcgttgaatg agagaaaaga aaagaaaagg aatgataaga gaaggaatga
     7081 aaattgtttt gttttgtttt caattgcttt cttttgtttt ctttgtcttt gttcttcctt
     7141 cccttgtccc gatgctgttc ttctcaccag cttccgcatg atagccattt tcttagttgt
     7201 tgtagggcat gcaagcttcc catggtgacg tcaccggttc tagataccta ggtaggcatt
     7261 ttgtttagtt aaaggtactt aagtaattag tataaacgaa ccaattttat aatcaggaag
     7321 ttaagtgaat ggtagcacat gtcgtaaaaa ttgtgaattt ttattgaata atattttaaa
     7381 tacaagcctt tctagactag gtatactcat aaacatatga gcaaaaggat agaggagatt
     7441 acattgcatc ttctacaaat ttatttattg ccctttactg aaaaattaaa taatgagtac
     7501 taaatgataa aaagcgctca gtacaagaaa gattgcaaaa atattgcatt cttcatgaat
     7561 taaagttgca tataaggcat attgaaagta atagtactaa aacagcagtt agcgaaaatt
     7621 aatagaatta tattcgcaag acaattgtga cattaaatta aaaaattgta gaatttttac
     7681 tatcctcttt aacgccatga gcctttataa aaaggttaaa ttagttttaa cattcttttt
     7741 ttgagtaaga gtttacaatt tatcaaaacc tgtgttatta tattcattag tttcaattta
     7801 ttagcatcta gagaaaaatc aattggcagt tacattgttg gaatttatga agaaaaagaa
     7861 tctacaacgg agaataagtt gctgatcgct ttccctaaaa ttgtatattt tgctgagctt
     7921 attttgacat ttcgttgaag ttttctctaa ttcgcattca ttttaagtaa aacaatgaga
     7981 aataaaatta caaaaaatac aaattaaaat acaattttta gctataatta tagacctagg
     8041 tgagctctgg taccctctag tcaaggcctt aagtgagtcg tattacggac tggccgtcgt
     8101 tttacaacgt cgtgactggg aaaaccctgg cgttacccaa cttaatcgcc ttgcagcaca
     8161 tccccctttc gccagctggc gtaatagcga agaggcccgc accgatcgcc cttcccaaca
     8221 gttgcgcagc ctgaatggcg aatggcgctt cgcttggtaa taaagcccgc ttcggcgggc
     8281 tttttttt
//
