LOCUS       sequence-72883-e         927 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Genetically encoded H2O2 probe for ratiometric and fluorescence 
            lifetime imaging.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 927)
  AUTHORS   Bilan DS, Pase L, Joosen L, Gorokhovatsky AY, Ermakova YG, Gadella 
            TW, Grabher C, Schultz C, Lukyanov S, Belousov VV
  TITLE     HyPer-3: a genetically encoded H2O2 probe with improved performance 
            for ratiometric and fluorescence lifetime imaging.
  JOURNAL   ACS Chem Biol. 2012 Dec 20.
  PUBMED    23256573
REFERENCE   2  (bases 1 to 927)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "ACS Chem 
            Biol. 2012 Dec 20."
COMMENT     Sequence Label: pQE-30-HyPer3
FEATURES             Location/Qualifiers
     source          1..927
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    15..31
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     protein_bind    47..63
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     RBS             82..93
                     /note="strong bacterial ribosome binding site (Elowitz and 
                     Leibler, 2000)"
     CDS             112..129
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     741..762
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     complement(871..892)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
ORIGIN
        1 aaaatttatt tgctttgtga gcggataaca attataatag attcaattgt gagcggataa
       61 caatttcaca cagaattcat taaagaggag aaattaacta tgagaggatc gcatcaccat
      121 caccatcacg gatccgagat ggcaagccag cagggcgaga cgatgtccgg accgctgcac
      181 attggtttga ttcccacagt tggaccgtac ctgctaccgc atattatccc tatgctgtac
      241 cagacctttc caaagctgga aatgtatctg catgaagcac agacccacca gttactggcg
      301 caactggaca gcggcaaact cgattgcgtg atcctcgcac tggtgaaaga gagcgaagca
      361 ttcattgaag tgccgttgtt tgatgagcca atgttgctgg ctatctatga agatcacccg
      421 tgggcgaacc gcgaatgcgt accgatggcc gatctggcag gggaaaaact gctgatgctg
      481 gaagatggtc actgtttgcg cgatcaggca atgtccgccg gctacaacag cgacaacgtc
      541 tatatcatgg ccgacaagca gaagaacggc atcaaggcca acttcaagat ccgccacaac
      601 gtcgaggacg gcagcgtgca gctcgccgac cactaccagc agaacacccc catcggcgac
      661 ggccccgtgc tgctgcccga caaccactac ctgagcttcc agtccgtcct gagcaaagac
      721 cccaacgaga agcgcgatca catggtcctg ctggagttcg tgaccgccgc cgggatcact
      781 ctcggcatgg acgagctgta caacgtggat ggcggtagcg gtggcaccgg cagcaagggc
      841 gaggagctgt tcaccggggt ggtgcccatc ctggtcgagc tggacggcga cgtaaacggc
      901 cacaagttca gcgtgtccgg cgagggc
//
