LOCUS       sequence-72895-e        1071 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  this report is the first to directly measure nAChr subunit 
            stoichiometry using FRET and plasma membrane localization of Alpha6 
            and Beta3 containing receptors using TIRF.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1071)
  AUTHORS   Drenan RM, Nashmi R, Imoukhuede P, Just H, McKinney S, Lester HA
  TITLE     Subcellular trafficking, pentameric assembly, and subunit 
            stoichiometry of neuronal nicotinic acetylcholine receptors 
            containing fluorescently labeled alpha6 and beta3 subunits.
  JOURNAL   Mol Pharmacol. 2008 Jan;73(1):27-41. Epub 2007 Oct 11.
  PUBMED    17932221
REFERENCE   2  (bases 1 to 1071)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol 
            Pharmacol."; date: "2008-01"; volume: "73(1)"; pages: "27-41. Epub 
            2007 Oct 11"
COMMENT     Sequence Label: pcDNA3.1-nAChr-Alpha6
FEATURES             Location/Qualifiers
     source          1..1071
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     61..80
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        61..79
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
ORIGIN
        1 ctatataagc agagctctct ggctaactag agaacccact gcttactggc ttatcgaaat
       61 taatacgact cactataggg agacccaagc tggctagcgt ttaaacttaa gcttggtacc
      121 gccaccatgc tgaacagccg ggaccaggga aacctgcact ccggtttatg tctgtggcta
      181 tgtggattcc tggctctctt taaaggcagt acaggctgtg aatctgaaga gcagctcttc
      241 cacaggctgt ttgctcacta caaccgcttc atccggccgg tggagaatgt ctccgatccc
      301 gtcacggtgc attttgaatt ggcaatcacg caactggcca atgtggatga agtcaaccag
      361 attatggaga ccaatctgtg gctgcgtcac atctggaagg actacagatt gcgttgggat
      421 ccaacggagt atgatggcat cgagacactt cgagttccag cagacaacat ctggaagcct
      481 gacatcgttc tgtataacaa tgctgtcggc gacttccagg tcgaaggcaa gaccaaagct
      541 cttctcaagt atgacggtgt gataacctgg accccaccag ccatctttaa gagctcctgc
      601 ccaatggaca tcaccttctt cccgtttgac catcaaaact gctccctgaa gtttggttcc
      661 tggacttatg acaaggcaga aatcgacctt ctcatcattg gctctaaagt agacatgaac
      721 gacttttggg aaaacagtga atgggaaatt gtcgacgcct ctggctataa gcatgacatc
      781 aagtacaact gctgtgaaga gatttacacg gacataacct actccttcta catcaggagg
      841 ttgcccatgt tttacaccat caacctcatc atcccctgcc tcttcatttc cttcctcacg
      901 gtgctggttt tttaccttcc ctccgactgt ggcgagaaag tgactctttg catctccgtt
      961 ctgctttctc tcactgtctt tttgctggtg attacagaga ccatcccatc cacatctctc
     1021 gtgatcccac tggtgggtga gtatctactg ttcaccatga tctttgtcac g
//
