LOCUS       sequence-72897-e        1037 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1037)
  AUTHORS   Cai D, Cohen KB, Luo T, Lichtman JW, Sanes JR
  TITLE     Improved tools for the Brainbow toolbox.
  JOURNAL   Nat Methods. 2013 May 5;10(6):540-7. doi: 10.1038/nmeth.2450. Epub 
            2013 May 5.
  PUBMED    23817127
REFERENCE   2  (bases 1 to 1037)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/nmeth.2450"; journalName: "Nat Methods"; date: "2013-05-5- 
            5"; volume: "10"; issue: "6"; pages: "540-7"
COMMENT     Sequence Label: pCAG-Autobow
FEATURES             Location/Qualifiers
     source          1..1037
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      626..635
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     protein_bind    651..684
                     /label=loxN
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (AGGTATAC) (Shaw et al., 2021). loxN sites are 
                     compatible with each other, but incompatible with loxP or 
                     lox2272 sites."
     polyA_signal    721..842
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(732..751)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     786..805
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
ORIGIN
        1 gtctctttgt cggcctcctt gattctttcc agtcttctgt ccacatagta gacgccgggc
       61 atcttgaggt tcttagcggg tttcttggat ctgtatgtgg tcttcaagtt gcagatcagg
      121 tggcccccgc ccacgagctt cagggccatg tcggctctgc cttccaggcc gccgtcagcg
      181 gggtacaggg tctcggtgga ggcctcccag ccgagtgttt tcttctgcat cacagggccg
      241 ttggatggga agttcacccc tctgatcttg acgttgtaga tgaggcagcc gtcctggagg
      301 ctggtgtcct gggtagcggt cagcacgccc ccgtcttcgt atgtggtgac tctctcccat
      361 gtgaagccct cggggaagga ctgcttaaag aagtcgggga tgccctgggt gtggttgatg
      421 aaggttttgc tgccgtacat gaagctggta gccaggatgt cgaaggcgaa ggggagaggg
      481 ccgccctcga ccgccttgat tctcatggtc tgggtgccct cgtagggctt gccttcgccc
      541 tcggatgtgc acttgaagtg gtggttgttc acggtgccct ccatgtacag cttcatgtgc
      601 atgttctcct taatcagctc gctcaccatg gtggcagtac tggatacttg ataacttcgt
      661 ataaggtata ctatacgaag ttatatgaag aacgcgaaga ttatgaagct cttcacgcct
      721 taagatacat tgatgagttt ggacaaacca caactagaat gcagtgaaaa aaatgcttta
      781 tttgtgaaat ttgtgatgct attgctttat ttgtaaccat tataagctgc aataaacaag
      841 ttaacaacaa caattgcatt cattttatgt ttcaggttca gggggaggtg tgggaggttt
      901 tttaaagcaa gtaaaacctc tacaaatgtg gtatggctga ttatgatcta gagtcgcggc
      961 cagatggagt tctgaggtca ttactggatc tatcaacagg agtccaagct cagctaatta
     1021 agcttcacag gtaggtc
//
