LOCUS       sequence-72898-e         927 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 927)
  AUTHORS   Cai D, Cohen KB, Luo T, Lichtman JW, Sanes JR
  TITLE     Improved tools for the Brainbow toolbox.
  JOURNAL   Nat Methods. 2013 May 5;10(6):540-7. doi: 10.1038/nmeth.2450. Epub 
            2013 May 5.
  PUBMED    23817127
REFERENCE   2  (bases 1 to 927)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/nmeth.2450"; journalName: "Nat Methods"; date: "2013-05-5- 
            5"; volume: "10"; issue: "6"; pages: "540-7"
COMMENT     Sequence Label: pCAG-Autobow
FEATURES             Location/Qualifiers
     source          1..927
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    27..51
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(100..133)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     protein_bind    complement(142..175)
                     /label=lox2272
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (AAGTATCC) (Shaw et al., 2021). lox2272 sites are 
                     compatible with each other, but incompatible with loxP or 
                     loxN sites (Lee and Saito, 1988)."
     protein_bind    complement(184..217)
                     /label=loxN
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (AGGTATAC) (Shaw et al., 2021). loxN sites are 
                     compatible with each other, but incompatible with loxP or 
                     lox2272 sites."
ORIGIN
        1 ttggcaaaga attcctcgag gatatcacaa gtttgtacaa aaaagcaggc tttaaaggaa
       61 cgcacgtaac tataacggtc ctaaggtagc gaacctagga taacttcgta tagcatacat
      121 tatacgaagt tatcggcgcg cataacttcg tataggatac tttatacgaa gttatcaagc
      181 gctataactt cgtatagtat accttatacg aagttatctg atgcaatggc caatttactg
      241 accgtacacc aaaatttgcc tgcattaccg gtcgatgcaa cgagtgatga ggttcgcaag
      301 aacctgatgg acatgttcag ggatcgccag gcgttttctg agcatacctg gaaaatgctt
      361 ctgtccgttt gccggtcgtg ggcggcatgg tgcaagttga ataaccggaa atggtttccc
      421 gcagaacctg aagatgttcg cgattatctt ctatatcttc aggcgcgcgg tctggcagta
      481 aaaactatcc agcaacattt gggccagcta aacatgcttc atcgtcggtc cgggctgcca
      541 cgaccaagtg acagcaatgc tgtttcactg gttatgcggc ggatccgaaa agaaaacgtt
      601 gatgccggtg aacgtgcaaa acaggctcta gcgttcgaac gcactgattt cgaccaggtt
      661 cgttcactca tggaaaatag cgatcgctgc caggatatac gtaatctggc atttctgggg
      721 attgcttata acaccctgtt acgtatagcc gaaattgcca ggatcagggt taaagatatc
      781 tcacgtactg acggtgggag aatgttaatc catattggca gaacgaaaac gctggttagc
      841 accgcaggtg tagagaaggc acttagcctg ggggtaacta aactggtcga gcgatggatt
      901 tccgtctctg gtgtagctga tgatccg
//
