LOCUS       sequence-72900-e        1078 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1078)
  AUTHORS   Cai D, Cohen KB, Luo T, Lichtman JW, Sanes JR
  TITLE     Improved tools for the Brainbow toolbox.
  JOURNAL   Nat Methods. 2013 May 5;10(6):540-7. doi: 10.1038/nmeth.2450. Epub 
            2013 May 5.
  PUBMED    23817127
REFERENCE   2  (bases 1 to 1078)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/nmeth.2450"; journalName: "Nat Methods"; date: "2013-05-5- 
            5"; volume: "10"; issue: "6"; pages: "540-7"
COMMENT     Sequence Label: pCAG-Autobow
FEATURES             Location/Qualifiers
     source          1..1078
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     polyA_signal    152..273
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(189..208)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     243..262
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     protein_bind    complement(290..323)
                     /label=lox2272
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (AAGTATCC) (Shaw et al., 2021). lox2272 sites are 
                     compatible with each other, but incompatible with loxP or 
                     loxN sites (Lee and Saito, 1988)."
     CDS             338..1042
                     /codon_start=1
                     /product="Phialidium yellow fluorescent proteim"
                     /label=PhiYFP
                     /note="mammalian codon-optimized"
                     /translation="MSSGALLFHGKIPYVVEMEGNVDGHTFSIRGKGYGDASVGKVDAQ
                     FICTTGDVPVPWSTLVTTLTYGAQCFAKYGPELKDFYKSCMPDGYVQERTITFEGDGNF
                     KTRAEVTFENGSVYNRVKLNGQGFKKDGHVLGKNLEFNFTPHCLYIWGDQANHGLKSAF
                     KICHEITGSKGDFIVADHTQMNTPIGGGPVHVPEYHHMSYHVKLSKDVTDHRDNMSLKE
                     TVRAVDCRKTYL"
ORIGIN
        1 ctctcggcat ggacgagctg tacaagtaaa gcggccgcga ctctagatca taatcagcca
       61 taccacattt gtagaggttt tacttgcttt aaaaaacctc ccacacctcc ccctgaacct
      121 gaaacataaa atgaatgcaa ttgttgttgt taacttgttt attgcagctt ataatggtta
      181 caaataaagc aatagcatca caaatttcac aaataaagca tttttttcac tgcattctag
      241 ttgtggtttg tccaaactca tcaatgtatc ttaaggcgtg tttaaacata taacttcgta
      301 taggatactt tatacgaagt tatcgcgtac ggccaccatg agcagcggcg ccctgctgtt
      361 ccacggcaag atcccctacg tggtggagat ggagggcaat gtggatggcc acaccttcag
      421 catccgcggc aagggctacg gcgatgccag cgtgggcaag gtggatgccc agttcatctg
      481 caccaccggc gatgtgcccg tgccctggag caccctggtg accaccctga cctacggcgc
      541 ccagtgcttc gccaagtacg gccccgagct gaaggatttc tacaagagct gcatgcccga
      601 tggctacgtg caggagcgca ccatcacctt cgagggtgat ggcaatttca agacccgcgc
      661 cgaggtgacc ttcgagaatg gcagcgtgta caatcgcgtg aagctgaatg gccagggctt
      721 caagaaggat ggccacgtgc tgggcaagaa tctggagttc aatttcaccc cccactgcct
      781 gtacatctgg ggcgatcagg ccaatcacgg cctgaagagc gccttcaaga tctgccacga
      841 gatcaccggc agcaagggcg atttcatcgt ggccgatcac acccagatga atacccccat
      901 cggcggcggc cccgtgcacg tgcccgagta ccaccacatg agctaccacg tgaagctgag
      961 caaggatgtg accgatcacc gcgataatat gagcctgaag gagaccgtgc gcgccgtgga
     1021 ttgccgcaag acctacctgt gaagcttaat tagctgagct tggactcctg ttgataga
//
