LOCUS       sequence-72903-e         865 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 865)
  AUTHORS   Cai D, Cohen KB, Luo T, Lichtman JW, Sanes JR
  TITLE     Improved tools for the Brainbow toolbox.
  JOURNAL   Nat Methods. 2013 May 5;10(6):540-7. doi: 10.1038/nmeth.2450. Epub 
            2013 May 5.
  PUBMED    23817127
REFERENCE   2  (bases 1 to 865)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/nmeth.2450"; journalName: "Nat Methods"; date: "2013-05-5- 
            5"; volume: "10"; issue: "6"; pages: "540-7"
COMMENT     Sequence Label: AAV-EF1a-BbTagBY
FEATURES             Location/Qualifiers
     source          1..865
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    complement(24..57)
                     /label=lox71
                     /bound_moiety="Cre recombinase"
                     /note="Left element (LE) mutant of loxP (Araki et al., 
                     2010). Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     regulatory      828..837
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 catttcaggt gtcgtgaggt acctaccgtt cgtatagcat acattatacg aagttattta
       61 ggagagcaca cacttgcagc tcatgcagcc ggggccactc tcatcaggag ggttcagctt
      121 actcacgcgt gcacttctgt gccccagttt gctagggagg tcgcagtatc tggccactgc
      181 cacctcgtgc tgctcgacgt aggtctcgtt gttggcctcc ttgattcttt ccagtctgta
      241 gtccacatag tagacgccag gcatcttgag gttcttagcg ggtttcttgg atctatatgt
      301 ggtcttgatg tttgcgatca gatggctccc gcccacgagc ttcagggcca tgtcgtttct
      361 gccttccagg ccgccgtcag cggggtacag cgtctcggtg aaggcctccc agccgagtgt
      421 tttcttctgc atcacagggc cgttggatgt gaagttcacc cctctgatct tgacgttgta
      481 gatgaggcag ccgtcctgga ggctggtgtc ctgggtagcg gtcagcacgc ccccgtcttc
      541 gtatgtggtg actctctccc atgtgaagcc ctcagggaag gactgcttga agaagtcggg
      601 gatgccctgg gtgtggttga tgaaggtctt gctgccgtag aggaagctag tagccaggat
      661 gtcgaaggcg aaggggagag ggccgccctc gaccaccttg attctcatgg tctgggtgcc
      721 ctcgtagggc ttgccttcgc cctcggatgt gcacttgaag tgatggttgt ccacggtgcc
      781 ctccatgtac agcttcatgt gcatgttctc cttaatcagc tcgctcacca tggtggcata
      841 acaaggtata atgtatgcta tacga
//
