LOCUS       sequence-72905-e         916 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 916)
  AUTHORS   Cai D, Cohen KB, Luo T, Lichtman JW, Sanes JR
  TITLE     Improved tools for the Brainbow toolbox.
  JOURNAL   Nat Methods. 2013 May 5;10(6):540-7. doi: 10.1038/nmeth.2450. Epub 
            2013 May 5.
  PUBMED    23817127
REFERENCE   2  (bases 1 to 916)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/nmeth.2450"; journalName: "Nat Methods"; date: "2013-05-5- 
            5"; volume: "10"; issue: "6"; pages: "540-7"
COMMENT     Sequence Label: AAV-EF1a-BbChT
FEATURES             Location/Qualifiers
     source          1..916
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    complement(57..90)
                     /label=loxKR3
                     /bound_moiety="Cre recombinase"
                     /note="Right element (RE) mutant of loxP (Araki et al., 
                     2010). Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     regulatory      91..100
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             97..804
                     /codon_start=1
                     /product="monomeric teal (cyan) fluorescent protein (Ai et 
                     al., 2006)"
                     /label=mTFP1
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEETTMGVIKPDMKIKLKMEGNVNGHAFVIEGEGEGKPYDG
                     TNTINLEVKEGAPLPFSYDILTTAFAYGNRAFTKYPDDIPNYFKQSFPEGYSWERTMTF
                     EDKGIVKVKSDISMEEDSFIYEIHLKGENFPPNGPVMQKKTTGWDASTERMYVRDGVLK
                     GDVKHKLLLEGGGHHRVDFKTIYRAKKAVKLPDYHFVDHRIEILNHDKDYNKVTVYESA
                     VARNSTDGMDELYK"
     protein_bind    883..916
                     /label=lox66
                     /bound_moiety="Cre recombinase"
                     /note="Right element (RE) mutant of loxP (Araki et al., 
                     2010). Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
ORIGIN
        1 gtcaatcttt cacaaatttt gtaatccaga ggttgattat cgataagctt gatatcataa
       61 cttcgtatag catacattat accttgttat gccaccatgg tgagcaaggg cgaggagacc
      121 acaatgggcg taatcaagcc cgacatgaag atcaagctga agatggaggg caacgtgaat
      181 ggccacgcct tcgtgatcga gggcgagggc gagggcaagc cctacgacgg caccaacacc
      241 atcaacctgg aggtgaagga gggagccccc ctgcccttct cctacgacat tctgaccacc
      301 gcgttcgcct acggcaacag ggccttcacc aagtaccccg acgacatccc caactacttc
      361 aagcagtcct tccccgaggg ctactcttgg gagcgcacca tgaccttcga ggacaagggc
      421 atcgtgaagg tgaagtccga catctccatg gaggaggact ccttcatcta cgagatacac
      481 ctcaagggcg agaacttccc ccccaacggc cccgtgatgc agaagaagac caccggctgg
      541 gacgcctcca ccgagaggat gtacgtgcgc gacggcgtgc tgaagggcga cgtcaagcac
      601 aagctgctgc tggagggcgg cggccaccac cgcgttgact tcaagaccat ctacagggcc
      661 aagaaggcgg tgaagctgcc cgactatcac tttgtggacc accgcatcga gatcctgaac
      721 cacgacaagg actacaacaa ggtgaccgtt tacgagagcg ccgtggcccg caactccacc
      781 gacggcatgg acgagctgta caagagtgca cgcgtgagta agctgaaccc tcctgatgag
      841 agtggccccg gctgcatgag ctgcaagtgt gtgctctcct gataccgttc gtataatgta
      901 tgctatacga agttat
//
