LOCUS       sequence-72906-e         944 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Hydrogen peroxide insensitive version of HyPerRed to be used as a pH
            control in HyPer experiments..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 944)
  AUTHORS   Ermakova YG, Bilan DS, Matlashov ME, Mishina NM, Markvicheva KN, 
            Subach OM, Subach FV, Bogeski I, Hoth M, Enikolopov G, Belousov VV
  TITLE     Red fluorescent genetically encoded indicator for intracellular 
            hydrogen peroxide.
  JOURNAL   Nat Commun. 2014 Oct 21;5:5222. doi: 10.1038/ncomms6222.
  PUBMED    25330925
REFERENCE   2  (bases 1 to 944)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2014-10-21"; volume: "5:5222. doi"; pages: " 
            10.1038/ncomms6222"
COMMENT     Sequence Label: pC1-HyPer-Red-C199S
FEATURES             Location/Qualifiers
     source          1..944
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      25..34
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 gttgaaccgt cagatccgct agccgccacc atggagatgg cgagccagca gggcgagacg
       61 atgtccggac cgctgcacat tggtttgatt cccacagttg gaccgtacct gctaccgcat
      121 attatcccta tgctgcacca gacctttcca aagctggaaa tgtatctgca tgaagcacag
      181 acccaccagt tactggcgca actggacagc ggcaaactcg attgcgtgat cctcgcgctg
      241 gtgaaagaga gcgaagcatt cattgaagtg ccgttgtttg atgagccaat gttgctggct
      301 atctatgaag atcacccgtg ggcgaaccgc gaatgcgtac cgatggccga tctggcaggg
      361 gaaaaactgc tgatgctgga agatggtcac tctttgcgcg atcaggcaat gccccgcacg
      421 gtttccgagc ggatgtaccc cgaggacggc gccctgaaga gcgagatcaa gaaggggctg
      481 aggctgaagg acggcggcca ctacgccgcc gaggtcaaga ccacctacaa ggccaagaag
      541 cccgtgcagc tgcccggcgc ctacatcgtc gacatcaagt tggacatcgt gtcccacaac
      601 gaggactaca ccatcgtgga acagtgcgaa cgcgccgagg gccgccactc caccggcggc
      661 atggacgagc tgtacaaggg aggtacaggc gggagtctgg tgagcaaggg cgaggaggat
      721 aacatggcca tcatcaagga gttcatgcgc ttcaaggtgc acatggaggg ctccgtgaac
      781 ggccacgagt tcgagatcga gggcgagggc gagggccgcc cctacgaggc ctttcagacc
      841 gctaagctga aggtgaccaa gggtggcccc ctgcccttcg cctgggacat cctgtcccct
      901 cagttcatgt acggctccaa ggcctacatt aagcacccag ccga
//
