LOCUS       sequence-72910-e        1069 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1069)
  AUTHORS   Cai D, Cohen KB, Luo T, Lichtman JW, Sanes JR
  TITLE     Improved tools for the Brainbow toolbox.
  JOURNAL   Nat Methods. 2013 May 5;10(6):540-7. doi: 10.1038/nmeth.2450. Epub 
            2013 May 5.
  PUBMED    23817127
REFERENCE   2  (bases 1 to 1069)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/nmeth.2450"; journalName: "Nat Methods"; date: "2013-05-5- 
            5"; volume: "10"; issue: "6"; pages: "540-7"
COMMENT     Sequence Label: pThy1-Brainbow3.2
FEATURES             Location/Qualifiers
     source          1..1069
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    171..195
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(244..277)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     protein_bind    complement(286..319)
                     /label=lox2272
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (AAGTATCC) (Shaw et al., 2021). lox2272 sites are 
                     compatible with each other, but incompatible with loxP or 
                     loxN sites (Lee and Saito, 1988)."
     protein_bind    complement(328..361)
                     /label=loxN
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (AGGTATAC) (Shaw et al., 2021). loxN sites are 
                     compatible with each other, but incompatible with loxP or 
                     lox2272 sites."
     regulatory      370..379
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             382..402
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
ORIGIN
        1 ccctaagaga aggggataaa gagaggggct gaggtattca tcatgtgctc cgtggatctc
       61 aagccctcaa ggtaaatggg gacccacctg tcctaccagc tggctgacct gtagctttcc
      121 ccaccacaga atccaagtcg gaactcttgg cacctagagg atctcgaatc acaagtttgt
      181 acaaaaaagc aggctttaaa ggaacgcacg taactataac ggtcctaagg tagcgaacct
      241 aggataactt cgtatagcat acattatacg aagttatcgg cgcgcataac ttcgtatagg
      301 atactttata cgaagttatc aagcgctata acttcgtata gtatacctta tacgaagtta
      361 tctgatatcg ccaccatggc tcctaagaag aagaggaagg tgatgagcag cggcgccctg
      421 ctgttccacg gcaagatccc ctacgtggtg gagatggagg gcaatgtgga tggccacacc
      481 ttcagcatcc gcggcaaggg ctacggcgat gccagcgtgg gcaaggtgga tgcccagttc
      541 atctgcacca ccggcgatgt gcccgtgccc tggagcaccc tggtgaccac cctgaccgca
      601 ggcgcccagt gcttcgccaa gtacggcccc gagctgaagg atttctacaa gagctgcatg
      661 cccgatggct acgtgcagga gcgcaccatc accttcgagg gtgatggcaa tttcaagacc
      721 cgcgccgagg tgaccttcga gaatggcagc gtgtacaatc gcgtgaagct gaatggccag
      781 ggcttcaaga aggatggcca cgtgctgggc aagaatctgg agttcaattt caccccccac
      841 tgcctgtaca tctggggcga tcaggccaat cacggcctga agagcgcctt caagatctgc
      901 cacgagatca ccggcagcaa gggcgatttc atcgtggccg atcacaccca gatgaatacc
      961 cccatcggcg gcggccccgt gcacgtgccc gagtaccacc acatgagcta ccacgtgaag
     1021 ctgagcaagg atgtgaccga tcaccgcgat aatatgagcc tgaaggaga
//
