LOCUS       sequence-72956-e        1049 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1049)
  AUTHORS   Cai D, Cohen KB, Luo T, Lichtman JW, Sanes JR
  TITLE     Improved tools for the Brainbow toolbox.
  JOURNAL   Nat Methods. 2013 May 5;10(6):540-7. doi: 10.1038/nmeth.2450. Epub 
            2013 May 5.
  PUBMED    23817127
REFERENCE   2  (bases 1 to 1049)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/nmeth.2450"; journalName: "Nat Methods"; date: "2013-05-5- 
            5"; volume: "10"; issue: "6"; pages: "540-7"
COMMENT     Sequence Label: pThy1-Brainbow3.2
FEATURES             Location/Qualifiers
     source          1..1049
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     225..246
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     regulatory      288..297
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     protein_bind    306..339
                     /label=lox2272
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (AAGTATCC) (Shaw et al., 2021). lox2272 sites are 
                     compatible with each other, but incompatible with loxP or 
                     loxN sites (Lee and Saito, 1988)."
     polyA_signal    356..477
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(367..386)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     421..440
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     CDS             717..728
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
ORIGIN
        1 gcgctcctgg acgtagcctt cgggcatggc ggacttgaag aagtcgtgct gcttcatgtg
       61 gtcggggtag cggctgaagc actgcacgcc gtaggtcagg gtggtcacga gggtgggcca
      121 gggcacgggc agcttgccgg tggtgcagat gaacttcagg gtcagcttgc cgtaggtggc
      181 atcgccctcg ccctcgccgg acacgctgaa cttgtggccg tttacgtcgc cgtccagctc
      241 gaccaggatg ggcaccaccc cggtgaacag ctcctcgccc ttgctcacca tggtggccgt
      301 acgcgataac ttcgtataaa gtatcctata cgaagttata tgtttaaaca cgccttaaga
      361 tacattgatg agtttggaca aaccacaact agaatgcagt gaaaaaaatg ctttatttgt
      421 gaaatttgtg atgctattgc tttatttgta accattataa gctgcaataa acaagttaac
      481 aacaacaatt gcattcattt tatgtttcag gttcaggggg aggtgtggga ggttttttaa
      541 agcaagtaaa acctctacaa atgtggtatg gctgattatg atctggtgca gcagtctcga
      601 cggtatcgat gcggggaggc ggcccaaagg gagatccgac tcgtctgagg gcgaaggcga
      661 agacgcggaa gaggccgcag agccggcagc aggccgcggg aaggaaggtc cgctggattg
      721 agggccgaag ggacgtagca gaaggacgtc ccgcgcagaa tccaggtggc aacacaggcg
      781 agcagccaag gaaaggacga tgatttcccc gacaacacca cggaattgtc agtgcccaac
      841 agccgagccc ctgtccagca gcgggcaagg caggcggcga tgagttccgc cgtggcaata
      901 gggaggggga aagcgaaagt cccggaaagg agctgacagg tggtggcaat gccccaacca
      961 gtgggggttg cgtcagcaaa cacagtgcac accacgccac gttgcctgac aacgggccac
     1021 aactcctcat aaagagacag caaccagga
//
