LOCUS       sequence-72957-e        1060 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1060)
  AUTHORS   Cai D, Cohen KB, Luo T, Lichtman JW, Sanes JR
  TITLE     Improved tools for the Brainbow toolbox.
  JOURNAL   Nat Methods. 2013 May 5;10(6):540-7. doi: 10.1038/nmeth.2450. Epub 
            2013 May 5.
  PUBMED    23817127
REFERENCE   2  (bases 1 to 1060)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/nmeth.2450"; journalName: "Nat Methods"; date: "2013-05-5- 
            5"; volume: "10"; issue: "6"; pages: "540-7"
COMMENT     Sequence Label: pThy1-Brainbow3.2
FEATURES             Location/Qualifiers
     source          1..1060
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      37..46
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     protein_bind    55..88
                     /label=loxN
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (AGGTATAC) (Shaw et al., 2021). loxN sites are 
                     compatible with each other, but incompatible with loxP or 
                     lox2272 sites."
     polyA_signal    107..228
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(118..137)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     172..191
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     misc_feature    362..950
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     CDS             468..479
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     877..897
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
ORIGIN
        1 gcttcatgtg catgttctcc ttaatcagct cgctcaccat ggtggcagta cttgataact
       61 tcgtataagg tatactatac gaagttatat gaagatcttc acgccttaag atacattgat
      121 gagtttggac aaaccacaac tagaatgcag tgaaaaaaat gctttatttg tgaaatttgt
      181 gatgctattg ctttatttgt aaccattata agctgcaata aacaagttaa caacaacaat
      241 tgcattcatt ttatgtttca ggttcagggg gaggtgtggg aggtttttta aagcaagtaa
      301 aacctctaca aatgtggtat ggctgattat gatctggtgc agcagtctcg acggtatcga
      361 tgcggggagg cggcccaaag ggagatccga ctcgtctgag ggcgaaggcg aagacgcgga
      421 agaggccgca gagccggcag caggccgcgg gaaggaaggt ccgctggatt gagggccgaa
      481 gggacgtagc agaaggacgt cccgcgcaga atccaggtgg caacacaggc gagcagccaa
      541 ggaaaggacg atgatttccc cgacaacacc acggaattgt cagtgcccaa cagccgagcc
      601 cctgtccagc agcgggcaag gcaggcggcg atgagttccg ccgtggcaat agggaggggg
      661 aaagcgaaag tcccggaaag gagctgacag gtggtggcaa tgccccaacc agtgggggtt
      721 gcgtcagcaa acacagtgca caccacgcca cgttgcctga caacgggcca caactcctca
      781 taaagagaca gcaaccagga tttatacaag gaggagaaaa tgaaagccat acgggaagca
      841 atagcatgat acaaaggcat taaagcagcg tatccacata gcgtaaaagg agcaacatag
      901 ttaagaatac cagtcaatct ttcacaaatt ttgtaatcca gaggttgatt agcggccgct
      961 caggagagca cacacttgca gctcatgcag ccggggccac tctcatcagg agggttcagc
     1021 ttactcacgc gtgcactctt gtacagctcg tccatgccga
//
