LOCUS       sequence-72959-e         820 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 820)
  AUTHORS   Cai D, Cohen KB, Luo T, Lichtman JW, Sanes JR
  TITLE     Improved tools for the Brainbow toolbox.
  JOURNAL   Nat Methods. 2013 May 5;10(6):540-7. doi: 10.1038/nmeth.2450. Epub 
            2013 May 5.
  PUBMED    23817127
REFERENCE   2  (bases 1 to 820)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/nmeth.2450"; journalName: "Nat Methods"; date: "2013-05-5- 
            5"; volume: "10"; issue: "6"; pages: "540-7"
COMMENT     Sequence Label: pThy1-Flpbow1
FEATURES             Location/Qualifiers
     source          1..820
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    171..195
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    202..235
                     /label=FRT3
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="supports FLP-mediated excision with other FRT3 sites
                     (Bosch et al., 2015)"
     protein_bind    244..277
                     /label=FRT (minimal)
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="supports FLP-mediated excision but not integration 
                     (Turan and Bode, 2011)"
     protein_bind    286..319
                     /label=FRT (minimal)
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="supports FLP-mediated excision but not integration 
                     (Turan and Bode, 2011)"
     regulatory      324..333
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     primer_bind     complement(463..481)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     731..750
                     /label=mCherry-F
                     /note="mCherry, forward primer"
ORIGIN
        1 ccctaagaga aggggataaa gagaggggct gaggtattca tcatgtgctc cgtggatctc
       61 aagccctcaa ggtaaatggg gacccacctg tcctaccagc tggctgacct gtagctttcc
      121 ccaccacaga atccaagtcg gaactcttgg cacctagagg atctcgaatc acaagtttgt
      181 acaaaaaagc aggctcctag ggaagttcct attcttcaaa tagtatagga acttccggcg
      241 cgcgaagttc ctattctcta gaaggtatag gaacttcagc gctaggaagt tcctattctc
      301 taaaaagtat aggaacttct atcgccacca tggtgagcaa gggcgaggag gtcatcaaag
      361 agttcatgcg cttcaaggtg cgcatggagg gctccatgaa cggccacgag ttcgagatcg
      421 agggcgaggg cgagggccgc ccctacgagg gcacccagac cgccaagctg aaggtgacca
      481 agggcggccc cctgcccttc gcctgggaca tcctgtcccc ccagttcatg tacggctcca
      541 aggcgtacgt gaagcacccc gccgacatcc ccgattacaa gaagctgtcc ttccccgagg
      601 gcttcaagtg ggagcgcgtg atgaacttcg aggacggcgg tctggtgacc gtgacccagg
      661 actcctccct gcaggacggc acgctgatct acaaggtgaa gatgcgcggc accaacttcc
      721 cccccgacgg ccccgtaatg cagaagaaga ccatgggctg ggaggcctcc accgagcgcc
      781 tgtacccccg cgacggcgtg ctgaagggcg agatccacca
//
