LOCUS       sequence-72960-e         897 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Transgenically expresses firefly luciferase protein in T. cruzi.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 897)
  AUTHORS   Canavaci AM, Bustamante JM, Padilla AM, Perez Brandan CM, Simpson 
            LJ, Xu D, Boehlke CL, Tarleton RL
  TITLE     In vitro and in vivo high-throughput assays for the testing of 
            anti-Trypanosoma cruzi compounds.
  JOURNAL   PLoS Negl Trop Dis. 2010 Jul 13;4(7):e740. doi: 
            10.1371/journal.pntd.0000740.
  PUBMED    20644616
REFERENCE   2  (bases 1 to 897)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS Negl 
            Trop Dis."; date: "2010-07-13"; volume: "4(7):e740. doi"; pages: " 
            10.1371/journal.pntd.0000740"
COMMENT     Sequence Label: pLew90-beta-GW/T7/PARP SAS/luciferase/Aldolase 3′UTR
FEATURES             Location/Qualifiers
     source          1..897
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     15..34
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        15..33
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    131..155
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     primer_bind     complement(227..245)
                     /label=LucNrev
                     /note="Firefly luciferase, reverse primer"
ORIGIN
        1 aagttgggag ctcgtaatac gactcactat agggcgaatt ggatcctgca cgcgccttcg
       61 agtttttttt ccttttcccc atttttttca acttgaagac ttcaattaca ccaaaaagta
      121 aaattcacaa gcaagtttgt acaaaaaagc aggctcaacc atggaagacg ccaaaaacat
      181 aaagaaaggc ccggcgccat tctatcctct agaggatgga accgctggag agcaactgca
      241 taaggctatg aagagatacg ccctggttcc tggaacaatt gcttttacag atgcacatat
      301 cgaggtgaac atcacgtacg cggaatactt cgaaatgtcc gttcggttgg cagaagctat
      361 gaaacgatat gggctgaata caaatcacag aatcgtcgta tgcagtgaaa actctcttca
      421 attctttatg ccggtgttgg gcgcgttatt tatcggagtt gcagttgcgc ccgcgaacga
      481 catttataat gaacgtgaat tgctcaacag tatgaacatt tcgcagccta ccgtagtgtt
      541 tgtttccaaa aaggggttgc aaaaaatttt gaacgtgcaa aaaaaattac caataatcca
      601 gaaaattatt atcatggatt ctaaaacgga ttaccaggga tttcagtcga tgtacacgtt
      661 cgtcacatct catctacctc ccggttttaa tgaatacgat tttgtaccag agtcctttga
      721 tcgtgacaaa acaattgcac tgataatgaa ttcctctgga tctactgggt tacctaaggg
      781 tgtggccctt ccgcatagaa ctgcctgcgt cagattctcg catgccagag atcctatttt
      841 tggcaatcaa atcattccgg atactgcgat tttaagtgtt gttccattcc atcacgg
//
