LOCUS       sequence-72961-e        1071 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1071)
  AUTHORS   Cai D, Cohen KB, Luo T, Lichtman JW, Sanes JR
  TITLE     Improved tools for the Brainbow toolbox.
  JOURNAL   Nat Methods. 2013 May 5;10(6):540-7. doi: 10.1038/nmeth.2450. Epub 
            2013 May 5.
  PUBMED    23817127
REFERENCE   2  (bases 1 to 1071)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/nmeth.2450"; journalName: "Nat Methods"; date: "2013-05-5- 
            5"; volume: "10"; issue: "6"; pages: "540-7"
COMMENT     Sequence Label: pThy1-Flpbow1
FEATURES             Location/Qualifiers
     source          1..1071
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     polyA_signal    123..244
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(160..179)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     214..233
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     protein_bind    259..292
                     /label=FRT (minimal)
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="supports FLP-mediated excision but not integration 
                     (Turan and Bode, 2011)"
     CDS             306..1010
                     /codon_start=1
                     /product="Phialidium yellow fluorescent proteim"
                     /label=PhiYFP
                     /note="mammalian codon-optimized"
                     /translation="MSSGALLFHGKIPYVVEMEGNVDGHTFSIRGKGYGDASVGKVDAQ
                     FICTTGDVPVPWSTLVTTLTYGAQCFAKYGPELKDFYKSCMPDGYVQERTITFEGDGNF
                     KTRAEVTFENGSVYNRVKLNGQGFKKDGHVLGKNLEFNFTPHCLYIWGDQANHGLKSAF
                     KICHEITGSKGDFIVADHTQMNTPIGGGPVHVPEYHHMSYHVKLSKDVTDHRDNMSLKE
                     TVRAVDCRKTYL"
ORIGIN
        1 agcggccgcg actctagatc ataatcagcc ataccacatt tgtagaggtt ttacttgctt
       61 taaaaaacct cccacacctc cccctgaacc tgaaacataa aatgaatgca attgttgttg
      121 ttaacttgtt tattgcagct tataatggtt acaaataaag caatagcatc acaaatttca
      181 caaataaagc atttttttca ctgcattcta gttgtggttt gtccaaactc atcaatgtat
      241 cttaaggcgt gtttaaacga agttcctatt ctctagaagg tataggaact tcccgtacgg
      301 ccaccatgag cagcggcgcc ctgctgttcc acggcaagat cccctacgtg gtggagatgg
      361 agggcaatgt ggatggccac accttcagca tccgcggcaa gggctacggc gatgccagcg
      421 tgggcaaggt ggatgcccag ttcatctgca ccaccggcga tgtgcccgtg ccctggagca
      481 ccctggtgac caccctgacc tacggcgccc agtgcttcgc caagtacggc cccgagctga
      541 aggatttcta caagagctgc atgcccgatg gctacgtgca ggagcgcacc atcaccttcg
      601 agggtgatgg caatttcaag acccgcgccg aggtgacctt cgagaatggc agcgtgtaca
      661 atcgcgtgaa gctgaatggc cagggcttca agaaggatgg ccacgtgctg ggcaagaatc
      721 tggagttcaa tttcaccccc cactgcctgt acatctgggg cgatcaggcc aatcacggcc
      781 tgaagagcgc cttcaagatc tgccacgaga tcaccggcag caagggcgat ttcatcgtgg
      841 ccgatcacac ccagatgaat acccccatcg gcggcggccc cgtgcacgtg cccgagtacc
      901 accacatgag ctaccacgtg aagctgagca aggatgtgac cgatcaccgc gataatatga
      961 gcctgaagga gaccgtgcgc gccgtggatt gccgcaagac ctacctgtga agcttaatta
     1021 gctgagcttg gactcctgtt gatagatcca gtaatgacct cagaactcca t
//
