LOCUS       sequence-72963-e        1023 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1023)
  AUTHORS   Cai D, Cohen KB, Luo T, Lichtman JW, Sanes JR
  TITLE     Improved tools for the Brainbow toolbox.
  JOURNAL   Nat Methods. 2013 May 5;10(6):540-7. doi: 10.1038/nmeth.2450. Epub 
            2013 May 5.
  PUBMED    23817127
REFERENCE   2  (bases 1 to 1023)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/nmeth.2450"; journalName: "Nat Methods"; date: "2013-05-5- 
            5"; volume: "10"; issue: "6"; pages: "540-7"
COMMENT     Sequence Label: pThy1-Flpbow1
FEATURES             Location/Qualifiers
     source          1..1023
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     225..246
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     regulatory      288..297
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     protein_bind    complement(305..338)
                     /label=FRT (minimal)
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="supports FLP-mediated excision but not integration 
                     (Turan and Bode, 2011)"
     polyA_signal    366..487
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(377..396)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     431..450
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
ORIGIN
        1 gcgctcctgg acgtagcctt cgggcatggc ggacttgaag aagtcgtgct gcttcatgtg
       61 gtcggggtag cgggcgaagc actgcacgcc ccaggtcagg gtggtcacga gggtgggcca
      121 gggcacgggc agcttgccgg tggtgcagat gaacttcagg gtcagcttgc cgtaggtggc
      181 atcgccctcg ccctcgccgg acacgctgaa cttgtggccg tttacgtcgc cgtccagctc
      241 gaccaggatg ggcaccaccc cggtgaacag ctcctcgccc ttgctcacca tggtggcagt
      301 actggaagtt cctatacttt ttagagaata ggaacttcct gaagattatg aagctcttca
      361 cgccttaaga tacattgatg agtttggaca aaccacaact agaatgcagt gaaaaaaatg
      421 ctttatttgt gaaatttgtg atgctattgc tttatttgta accattataa gctgcaataa
      481 acaagttaac aacaacaatt gcattcattt tatgtttcag gttcaggggg aggtgtggga
      541 ggttttttaa agcaagtaaa acctctacaa atgtggtatg gctgattatg atctagagtc
      601 gcggccagat ggagttctga ggtcattact ggatctatca acaggagtcc aagctcagct
      661 aattaagctt cacaggtagg tcttgcggca atccacggcg cgcacggtct ccttcaggct
      721 catattatcg cggtgatcgg tcacatcctt gctcagcttc acgtggtagc tcatgtggtg
      781 gtactcgggc acgtgcacgg ggccgccgcc gatgggggta ttcatctggg tgtgatcggc
      841 cacgatgaaa tcgcccttgc tgccggtgat ctcgtggcag atcttgaagg cgctcttcag
      901 gccgtgattg gcctgatcgc cccagatgta caggcagtgg ggggtgaaat tgaactccag
      961 attcttgccc agcacgtggc catccttctt gaagccctgg ccattcagct tcacgcgatt
     1021 gta
//
