LOCUS       sequence-72969-e         955 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 955)
  AUTHORS   Cai D, Cohen KB, Luo T, Lichtman JW, Sanes JR
  TITLE     Improved tools for the Brainbow toolbox.
  JOURNAL   Nat Methods. 2013 May 5;10(6):540-7. doi: 10.1038/nmeth.2450. Epub 
            2013 May 5.
  PUBMED    23817127
REFERENCE   2  (bases 1 to 955)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/nmeth.2450"; journalName: "Nat Methods"; date: "2013-05-5- 
            5"; volume: "10"; issue: "6"; pages: "540-7"
COMMENT     Sequence Label: pThy1-Flpbow3
FEATURES             Location/Qualifiers
     source          1..955
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    206..239
                     /label=FRT3
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="supports FLP-mediated excision with other FRT3 sites
                     (Bosch et al., 2015)"
     protein_bind    290..323
                     /label=FRT (minimal)
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="supports FLP-mediated excision but not integration 
                     (Turan and Bode, 2011)"
     regulatory      330..339
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             342..362
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
ORIGIN
        1 ctccctaaga gaaggggata aagagagggg ctgaggtatt catcatgtgc tccgtggatc
       61 tcaagccctc aaggtaaatg gggacccacc tgtcctacca gctggctgac ctgtagcttt
      121 ccccaccaca gaatccaagt cggaactctt ggcacctaga ggatctcgag cgtaactata
      181 acggtcctaa ggtagcgaac ctagggaagt tcctattctt caaatagtat aggaacttcc
      241 ggcgcgcgaa gttcctattc tcnagaaggt ataggaactt cagcgctagg aagttcctat
      301 tctctaaaaa gtataggaac ttctacatcg ccaccatggc tcctaagaag aagaggaagg
      361 tgatgagcag cggcgccctg ctgttccacg gcaagatccc ctacgtggtg gagatggagg
      421 gcaatgtgga tggccacacc ttcagcatcc gcggcaaggg ctacggcgat gccagcgtgg
      481 gcaaggtgga tgcccagttc atctgcacca ccggcgatgt gcccgtgccc tggagcaccc
      541 tggtgaccac cctgaccgca ggcgcccagt gcttcgccaa gtacggcccc gagctgaagg
      601 atttctacaa gagctgcatg cccgatggct acgtgcagga gcgcaccatc accttcgagg
      661 gtgatggcaa tttcaagacc cgcgccgagg tgaccttcga gaatggcagc gtgtacaatc
      721 gcgtgaagct gaatggccag ggcttcaaga aggatggcca cgtgctgggc aagaatctgg
      781 agttcaattt caccccccac tgcctgtaca tctggggcga tcaggccaat cacggcctga
      841 agagcgcctt caagatctgc cacgagatca ccggcagcaa gggcgatttc atcgtggccg
      901 atcacaccca gatgaatacc cccatcggcg gcggccccgt gcacgtgccc gagta
//
