LOCUS       sequence-72971-e         948 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 948)
  AUTHORS   Cai D, Cohen KB, Luo T, Lichtman JW, Sanes JR
  TITLE     Improved tools for the Brainbow toolbox.
  JOURNAL   Nat Methods. 2013 May 5;10(6):540-7. doi: 10.1038/nmeth.2450. Epub 
            2013 May 5.
  PUBMED    23817127
REFERENCE   2  (bases 1 to 948)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/nmeth.2450"; journalName: "Nat Methods"; date: "2013-05-5- 
            5"; volume: "10"; issue: "6"; pages: "540-7"
COMMENT     Sequence Label: pThy1-Flpbow3
FEATURES             Location/Qualifiers
     source          1..948
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     225..246
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     regulatory      603..612
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     protein_bind    complement(620..653)
                     /label=FRT (minimal)
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="supports FLP-mediated excision but not integration 
                     (Turan and Bode, 2011)"
     polyA_signal    668..789
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(679..698)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     733..752
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
ORIGIN
        1 gcgctcctgg acgtagcctt cgggcatggc ggacttgaag aagtcgtgct gcttcatgtg
       61 gtcggggtag cggctgaagc actgcacgcc gtaggtcagg gtggtcacga gggtgggcca
      121 gggcacgggc agcttgccgg tggtgcagat gaacttcagg gtcagcttgc cgtaggtggc
      181 atcgccctcg ccctcgccgg acacgctgaa cttgtggccg tttacgtcgc cgtccagctc
      241 gaccaggatg ggcaccaccc cggtgaacag ctcctcgccc ttgctcacca tagcgcctgc
      301 gcttccgccg atctgctccc tgtgggcctc gatgatgtcg ttgtcctcca tgtccaggtc
      361 ctcgggggtc tggtcggcct ggatctcgat gccgtcgtac aggaaggtca ggctgtccat
      421 ctccttgccc tgcctcttgg cgaaggcctc catcagcctc ctcagggggg tggtcttctt
      481 gatcttgaag aagatctcgc tgctgccgtc gctcaccttc aggttgatgt gggtctctgg
      541 cttgacttca ggtttcacct cgggcttggc ctcctggttc acctcgctgt cctgcaggct
      601 gcccatggtg gccgtacggg aagttcctat accttctaga gaataggaac ttcgtttaaa
      661 cacgccttaa gatacattga tgagtttgga caaaccacaa ctagaatgca gtgaaaaaaa
      721 tgctttattt gtgaaatttg tgatgctatt gctttatttg taaccattat aagctgcaat
      781 aaacaagtta acaacaacaa ttgcattcat tttatgtttc aggttcaggg ggaggtgtgg
      841 gaggtttttt aaagcaagta aaacctctac aaatgtggta tggctgatta tgatctagag
      901 tcgcggccgc tcaggagagc acacacttgc agctcatgca gccggggc
//
