LOCUS       sequence-72972-e         921 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Gateway adapted transfer of Hygromycin resistance gene.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 921)
  AUTHORS   Xu D, Brandan CP, Basombrio MA, Tarleton RL
  TITLE     Evaluation of high efficiency gene knockout strategies for 
            Trypanosoma cruzi.
  JOURNAL   BMC Microbiol. 2009 May 11;9:90. doi: 10.1186/1471-2180-9-90.
  PUBMED    19432966
REFERENCE   2  (bases 1 to 921)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "BMC 
            Microbiol."; date: "2009-05-11"; volume: "9:90. doi"; pages: " 
            10.1186/1471-2180-9-90"
COMMENT     Sequence Label: pDONR221-Hyg
FEATURES             Location/Qualifiers
     source          1..921
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    70..94
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
ORIGIN
        1 atgattttat tttgactgat agtgacctgt tcgttgcaac aaattgatga gcaatgcttt
       61 tttataatgc caactttgta caaaaaagca ggctatgaaa aagcctgaac tcaccgcgac
      121 gtctgtcgag aagtttctga tcgaaaagtt cgacagcgtc tccgacctga tgcagctctc
      181 ggagggcgaa gaatctcgtg ctttcagctt cgatgtagga gggcgtggat atgtcctgcg
      241 ggtaaatagc tgcgccgatg gtttctacaa agatcgttat gtttatcggc actttgcatc
      301 ggccgcgctc ccgattccgg aagtgcttga cattggggaa ttcagcgaga gcctgaccta
      361 ttgcatctcc cgccgtgcac agggtgtcac gttgcaagac ctgcctgaaa ccgaactgcc
      421 cgctgttctg cagccggtcg cggaggccat ggatgcgatc gctgcggccg atcttagcca
      481 gacgagcggg ttcggcccat tcggaccgca aggaatcggt caatacacta catggcgtga
      541 tttcatatgc gcgattgctg atccccatgt gtatcactgg caaactgtga tggacgacac
      601 cgtcagtgcg tccgtcgcgc aggctctcga tgagctgatg ctttgggccg aggactgccc
      661 cgaagtccgg cacctcgtgc acgcggattt cggctccaac aatgtcctga cggacaatgg
      721 ccgcataaca gcggtcattg actggagcga ggcgatgttc ggggattccc aatacgaggt
      781 cgccaacatc ttcttctgga ggccgtggtt ggcttgtatg gagcagcaga cgcgctactt
      841 cgagcggagg catccggagc ttgcaggatc gccgcggctc cgggcgtata tgctccgcat
      901 tggtcttgac caactctatc a
//
