LOCUS       sequence-72973-e         995 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 995)
  AUTHORS   Cai D, Cohen KB, Luo T, Lichtman JW, Sanes JR
  TITLE     Improved tools for the Brainbow toolbox.
  JOURNAL   Nat Methods. 2013 May 5;10(6):540-7. doi: 10.1038/nmeth.2450. Epub 
            2013 May 5.
  PUBMED    23817127
REFERENCE   2  (bases 1 to 995)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/nmeth.2450"; journalName: "Nat Methods"; date: "2013-05-5- 
            5"; volume: "10"; issue: "6"; pages: "540-7"
COMMENT     Sequence Label: pThy1-Flpbow3
FEATURES             Location/Qualifiers
     source          1..995
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      352..361
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     protein_bind    complement(369..402)
                     /label=FRT (minimal)
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="supports FLP-mediated excision but not integration 
                     (Turan and Bode, 2011)"
     polyA_signal    421..542
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(432..451)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     486..505
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     complement(784..805)
                     /label=EGFP-C
                     /note="EGFP, forward primer"
ORIGIN
        1 gcttcatgtg catgttctcc ttaatcagct cgctcaccat agcgcctgcg cttccgccga
       61 tctgctccct gtgggcctcg atgatgtcgt tgtcctccat gtccaggtcc tcgggggtct
      121 ggtcggcctg gatctcgatg ccgtcgtaca ggaaggtcag gctgtccatc tccttgccct
      181 gcctcttggc gaaggcctcc atcagcctcc tcaggggggt ggtcttcttg atcttgaaga
      241 agatctcgct gctgccgtcg ctcaccttca ggttgatgtg ggtctctggc ttgacttcag
      301 gtttcacctc gggcttggcc tcctggttca cctcgctgtc ctgcaggctg cccatggtgg
      361 cagtactgga agttcctata ctttttagag aataggaact tcctgaagat cttcacgcct
      421 taagatacat tgatgagttt ggacaaacca caactagaat gcagtgaaaa aaatgcttta
      481 tttgtgaaat ttgtgatgct attgctttat ttgtaaccat tataagctgc aataaacaag
      541 ttaacaacaa caattgcatt cattttatgt ttcaggttca gggggaggtg tgggaggttt
      601 tttaaagcaa gtaaaacctc tacaaatgtg gtatggctga ttatgatcta gagtcgcggc
      661 cgctcaggag agcacacact tgcagctcat gcagccgggg ccactctcat caggagggtt
      721 cagcttactt acgcgtgcac tcttgtacag ctcgtccatg ccgagagtga tcccggcggc
      781 ggtcacgaac tccagcagga ccatgtgatc gcgcttctcg ttggggtctt tgctcagggc
      841 ggactgggtg ctcaggtagt ggttgtcggg cagcagcacg gggccgtcgc cgatgggggt
      901 gttctgctgg tagtggtcgg cgagctgcac gctgccgtcc tcgatgttgt ggcggatctt
      961 gaagttcacc ttgatgccgt tcttctgctt gtcgg
//
