LOCUS       sequence-72974-e        1038 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1038)
  AUTHORS   Mon H, Sugahara R, Hong SM, Lee JM, Kamachi Y, Kawaguchi Y, Kusakabe
            T
  TITLE     Analysis of protein interactions with two-hybrid system in cultured 
            insect cells.
  JOURNAL   Anal Biochem. 2009 Sep 15;392(2):180-2. doi: 
            10.1016/j.ab.2009.05.033.
  PUBMED    19481053
REFERENCE   2  (bases 1 to 1038)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.ab.2009.05"; journalName: "Anal Biochem"; date: 
            "2009-09-15- 15"; volume: "392"; issue: "2"; pages: "180-2"
COMMENT     Sequence Label: pIE2-AD
FEATURES             Location/Qualifiers
     source          1..1038
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             71..91
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     regulatory      101..110
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             110..175
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
     CDS             197..739
                     /codon_start=1
                     /product="C-terminal portion of the p65 subunit of mouse 
                     NF-kappa-B"
                     /label=p65
                     /translation="PSGQISNQALALAPSSAPVLAQTMVPSSAMVPLAQPPAPAPVLTP
                     GPPQSLSAPVPKSTQAGEGTLSEALLHLQFDADEDLGALLGNSTDPGVFTDLASVDNSE
                     FQQLLNQGVSMSHSTAEPMLMEYPEAITRLVTGSQRPPDPAPTPLGTSGLPNGLSGDED
                     FSSIADMDFSALLSQISS"
     protein_bind    749..873
                     /gene="mutant version of attR"
                     /label=attR1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     promoter        join(898..903,904..921,922..928)
                     /label=lac UV5 promoter
                     /note="E. coli lac promoter with an ""up"" mutation"
ORIGIN
        1 tctgttcgaa tttaaagctt ggtaccgaga tcgaggcctg tctagagaag cttgttcgaa
       61 tctcgagatg cctaaaaaga agcgtaaagt cgagtactcc gccaccatgg actacaaaga
      121 ccatgacggt gattataaag atcatgacat cgattacaag gatgacgatg acaagcaccg
      181 gtatactagt gaattccctt ctgggcaaat ctcaaaccag gccctggcct tagcaccgtc
      241 ctctgcccca gtccttgccc agaccatggt cccttcctca gccatggtac ctctggctca
      301 gcccccagct cctgccccag ttctaacccc gggtcctccc cagtccctgt ctgcacctgt
      361 tccaaagagc acccaggctg gggaaggcac gctgtcggaa gccctgctgc acctgcagtt
      421 tgatgctgat gaagacttgg gggccttgct tggcaacagc acagacccag gagtgttcac
      481 agacctggca tctgtggaca actcagagtt tcagcagctc ctgaaccagg gtgtgtccat
      541 gtctcactcc acagctgagc ccatgctgat ggagtaccct gaagctataa ctcgcctggt
      601 gacagggtcc cagaggcccc ctgacccagc tcccacaccc ctggggacct cggggcttcc
      661 caatggtctc tccggagatg aagacttctc ctccattgcg gacatggact tctctgctct
      721 gctgagtcag atcagctcca gcggccaaac aagtttgtac aaaaaagctg aacgagaaac
      781 gtaaaatgat ataaatatca atatattaaa ttagattttg cataaaaaac agactacata
      841 atactgtaaa acacaacata tccagtcact atggcggccg cattaggcac cccaggcttt
      901 acactttatg cttccggctc gtataatgtg tggattttga gttaggatcc gtcgagattt
      961 tcaggagcta aggaagctaa aatggagaaa aaaatcactg gatataccac cgttgatata
     1021 tcccaatggc atcgtaaa
//
