LOCUS       sequence-72975-e        1033 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1033)
  AUTHORS   Mon H, Sugahara R, Hong SM, Lee JM, Kamachi Y, Kawaguchi Y, Kusakabe
            T
  TITLE     Analysis of protein interactions with two-hybrid system in cultured 
            insect cells.
  JOURNAL   Anal Biochem. 2009 Sep 15;392(2):180-2. doi: 
            10.1016/j.ab.2009.05.033.
  PUBMED    19481053
REFERENCE   2  (bases 1 to 1033)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.ab.2009.05"; journalName: "Anal Biochem"; date: 
            "2009-09-15- 15"; volume: "392"; issue: "2"; pages: "180-2"
COMMENT     Sequence Label: pIE2-AD
FEATURES             Location/Qualifiers
     source          1..1033
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        complement(join(106..112,113..130,131..136))
                     /label=lac UV5 promoter
                     /note="E. coli lac promoter with an ""up"" mutation"
     protein_bind    complement(161..285)
                     /gene="mutant version of attR"
                     /label=attR1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     CDS             complement(295..837)
                     /codon_start=1
                     /product="C-terminal portion of the p65 subunit of mouse 
                     NF-kappa-B"
                     /label=p65
                     /translation="PSGQISNQALALAPSSAPVLAQTMVPSSAMVPLAQPPAPAPVLTP
                     GPPQSLSAPVPKSTQAGEGTLSEALLHLQFDADEDLGALLGNSTDPGVFTDLASVDNSE
                     FQQLLNQGVSMSHSTAEPMLMEYPEAITRLVTGSQRPPDPAPTPLGTSGLPNGLSGDED
                     FSSIADMDFSALLSQISS"
     CDS             complement(859..924)
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
     regulatory      924..933
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             complement(943..963)
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
ORIGIN
        1 gatgccattg ggatatatca acggtggtat atccagtgat ttttttctcc attttagctt
       61 ccttagctcc tgaaaatctc gacggatcct aactcaaaat ccacacatta tacgagccgg
      121 aagcataaag tgtaaagcct ggggtgccta atgcggccgc catagtgact ggatatgttg
      181 tgttttacag tattatgtag tctgtttttt atgcaaaatc taatttaata tattgatatt
      241 tatatcattt tacgtttctc gttcagcttt tttgtacaaa cttgtttggc cgctggagct
      301 gatctgactc agcagagcag agaagtccat gtccgcaatg gaggagaagt cttcatctcc
      361 ggagagacca ttgggaagcc ccgaggtccc caggggtgtg ggagctgggt cagggggcct
      421 ctgggaccct gtcaccaggc gagttatagc ttcagggtac tccatcagca tgggctcagc
      481 tgtggagtga gacatggaca caccctggtt caggagctgc tgaaactctg agttgtccac
      541 agatgccagg tctgtgaaca ctcctgggtc tgtgctgttg ccaagcaagg cccccaagtc
      601 ttcatcagca tcaaactgca ggtgcagcag ggcttccgac agcgtgcctt ccccagcctg
      661 ggtgctcttt ggaacaggtg cagacaggga ctggggagga cccggggtta gaactggggc
      721 aggagctggg ggctgagcca gaggtaccat ggctgaggaa gggaccatgg tctgggcaag
      781 gactggggca gaggacggtg ctaaggccag ggcctggttt gagatttgcc cagaagggaa
      841 ttcactagta taccggtgct tgtcatcgtc atccttgtaa tcgatgtcat gatctttata
      901 atcaccgtca tggtctttgt agtccatggt ggcggagtac tcgactttac gcttcttttt
      961 aggcatctcg agattcgaac aagcttctct agacaggcct cgatctcggt accaagcttt
     1021 aaattcgaac aga
//
