LOCUS       sequence-72976-e        1061 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1061)
  AUTHORS   Mon H, Sugahara R, Hong SM, Lee JM, Kamachi Y, Kawaguchi Y, Kusakabe
            T
  TITLE     Analysis of protein interactions with two-hybrid system in cultured 
            insect cells.
  JOURNAL   Anal Biochem. 2009 Sep 15;392(2):180-2. doi: 
            10.1016/j.ab.2009.05.033.
  PUBMED    19481053
REFERENCE   2  (bases 1 to 1061)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.ab.2009.05"; journalName: "Anal Biochem"; date: 
            "2009-09-15- 15"; volume: "392"; issue: "2"; pages: "180-2"
COMMENT     Sequence Label: pIE2-AD
FEATURES             Location/Qualifiers
     source          1..1061
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    complement(132..256)
                     /gene="mutant version of attR"
                     /label=attR2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     CDS             286..327
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     CDS             337..354
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     polyA_signal    372..501
                     /gene="immediate-early gene 2 from the Orgyia pseudotsugata
                     multicapsid nucleopolyhedrovirus"
                     /label=OpIE-2 poly(A) signal
                     /note="baculovirus polyadenylation signal"
     primer_bind     complement(709..728)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 ggtctccgtt atcggggaag aagtggctga tctcagccac cgcgaaaatg acatcaaaaa
       61 cgccattaac ctgatgttct ggggaatata aatgtcaggc tcccttatac acagccagtc
      121 tgcaggtcga ccatagtgac tggatatgtt gtgttttaca gtattatgta gtctgttttt
      181 tatgcaaaat ctaatttaat atattgatat ttatatcatt ttacgtttct cgttcagctt
      241 tcttgtacaa agtggtgacg taagctagag ggcccgcggt tcgaaggtaa gcctatccct
      301 aaccctctcc tcggtctcga ttctacgcgt accggtcatc atcaccatca ccattgagtt
      361 tatctgacta aatcttagtt tgtattgtca tgttttaata caatatgtta tgtttaaata
      421 tgtttttaat aaattttata aaataatttc aacttttatt gtaacaacat tgtccattta
      481 cacactcctt tcaagcgcgt gggatcgatg ctcactcaaa ggcggtaata cggttatcca
      541 cagaatcagg ggataacgca ggaaagaaca tgtgagcaaa aggccagcaa aaggccagga
      601 accgtaaaaa ggccgcgttg ctggcgtttt tccataggct ccgcccccct gacgagcatc
      661 acaaaaatcg acgctcaagt cagaggtggc gaaacccgac aggactataa agataccagg
      721 cgtttccccc tggaagctcc ctcgtgcgct ctcctgttcc gaccctgccg cttaccggat
      781 acctgtccgc ctttctccct tcgggaagcg tggcgctttc tcatagctca cgctgtaggt
      841 atctcagttc ggtgtaggtc gttcgctcca agctgggctg tgtgcacgaa ccccccgttc
      901 agcccgaccg ctgcgcctta tccggtaact atcgtcttga gtccaacccg gtaagacacg
      961 acttatcgcc actggcagca gccactggta acaggattag cagagcgagg tatgtaggcg
     1021 gtgctacaga gttcttgaag tggtggccta actacggcta c
//
