LOCUS       sequence-72986-e         745 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses CAS9 nuclease in mammalian cells. Used to modify genome of
            mouse embroys.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 745)
  AUTHORS   Fujii W, Kawasaki K, Sugiura K, Naito K
  TITLE     Efficient generation of large-scale genome-modified mice using gRNA 
            and CAS9 endonuclease.
  JOURNAL   Nucleic Acids Res. 2013 Aug 30.
  PUBMED    23997119
REFERENCE   2  (bases 1 to 745)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nucleic 
            Acids Res. 2013 Aug 30."
COMMENT     Sequence Label: pCAG-T3-hCAS-pA
FEATURES             Location/Qualifiers
     source          1..745
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             6..26
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
ORIGIN
        1 tggccccaaa gaagaagcgg aaggtcggta tccacggagt cccagcagcc gacaagaagt
       61 actccattgg gctcgatatc ggcacaaaca gcgtcggctg ggccgtcatt acggacgagt
      121 acaaggtgcc gagcaaaaaa ttcaaagttc tgggcaatac cgatcgccac agcataaaga
      181 agaacctcat tggcgccctc ctgttcgact ccggggagac ggccgaagcc acgcggctca
      241 aaagaacagc acggcgcaga tatacccgca gaaagaatcg gatctgctac ctgcaggaga
      301 tctttagtaa tgagatggct aaggtggatg actctttctt ccataggctg gaggagtcct
      361 ttttggtgga ggaggataaa aagcacgagc gccacccaat ctttggcaat atcgtggacg
      421 aggtggcgta ccatgaaaag tacccaacca tatatcatct gaggaagaag cttgtagaca
      481 gtactgataa ggctgacttg cggttgatct atctcgcgct ggcgcatatg atcaaatttc
      541 ggggacactt cctcatcgag ggggacctga acccagacaa cagcgatgtc gacaaactct
      601 ttatccaact ggttcagact tacaatcagc ttttcgaaga gaacccgatc aacgcatccg
      661 gagttgacgc caaagcaatc ctgagcgcta ggctgtccaa atcccggcgg ctcgaaaacc
      721 tcatcgcaca gctccctggg gagaa
//
