LOCUS       sequence-72991-e        1030 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Contributes the coding sequence for the mKate2 fluorescent protein 
            as the 5'-module during MultiSite Gateway-cloning of chimeric cDNAs 
            encoding three-part fusion proteins..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1030)
  AUTHORS   Buj R, Iglesias N, Planas AM, Santalucia T
  TITLE     A plasmid toolkit for cloning chimeric cDNAs encoding customized 
            fusion proteins into any Gateway destination expression vector.
  JOURNAL   BMC Mol Biol. 2013 Aug 20;14(1):18. doi: 10.1186/1471-2199-14-18.
  PUBMED    23957834
REFERENCE   2  (bases 1 to 1030)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "BMC Mol 
            Biol."; date: "2013-08-20"; volume: "14(1):18. doi"; pages: " 
            10.1186/1471-2199-14-18"
COMMENT     Sequence Label: pDONR P4-P1R-mKate2
FEATURES             Location/Qualifiers
     source          1..1030
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    29..124
                     /gene="mutant version of attL"
                     /label=attL4
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     regulatory      127..136
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             133..828
                     /codon_start=1
                     /product="monomeric far-red fluorescent protein (Shcherbo 
                     et al., 2009)"
                     /label=mKate2
                     /note="mammalian codon-optimized"
                     /translation="MVSELIKENMHMKLYMEGTVNNHHFKCTSEGEGKPYEGTQTMRIK
                     AVEGGPLPFAFDILATSFMYGSKTFINHTQGIPDFFKQSFPEGFTWERVTTYEDGGVLT
                     ATQDTSLQDGCLIYNVKIRGVNFPSNGPVMQKKTLGWEASTETLYPADGGLEGRADMAL
                     KLVGGGHLICNLKTTYRSKKPAKNLKMPGVYYVDRRLERIKEADKETYVEQHEVAVARY
                     CDLPSKLGHR"
     misc_feature    841..906
                     /label=MCS
                     /note="multiple cloning site"
ORIGIN
        1 gggccgagtt acgctaccat ggagctccaa ataatgattt tattttgact gatagtgacc
       61 tgttcgttgc aacaaattga taagcaatgc ttttttataa tgccaacttt gtatagaaaa
      121 gttgccgcca ccatggtgag cgagctgatt aaggagaaca tgcacatgaa gctgtacatg
      181 gagggcaccg tgaacaacca ccacttcaag tgcacatccg agggcgaagg caagccctac
      241 gagggcaccc agaccatgag aatcaaggcg gtcgagggcg gccctctccc cttcgccttc
      301 gacatcctgg ctaccagctt catgtacggc agcaaaacct tcatcaacca cacccagggc
      361 atccccgact tctttaagca gtccttcccc gagggcttca catgggagag agtcaccaca
      421 tacgaagacg ggggcgtgct gaccgctacc caggacacca gcctccagga cggctgcctc
      481 atctacaacg tcaagatcag aggggtgaac ttcccatcca acggccctgt gatgcagaag
      541 aaaacactcg gctgggaggc ctccaccgag accctgtacc ccgctgacgg cggcctggaa
      601 ggcagagccg acatggccct gaagctcgtg ggcgggggcc acctgatctg caacttgaag
      661 accacataca gatccaagaa acccgctaag aacctcaaga tgcccggcgt ctactatgtg
      721 gacagaagac tggaaagaat caaggaggcc gacaaagaga cctacgtcga gcagcacgag
      781 gtggctgtgg ccagatactg cgacctccct agcaaactgg ggcacagagg tggaggaggt
      841 tccggactca gatctcgagc tcaagcttcg aattctgcag tcgacggtac cgcgggcccg
      901 ggatccaccg gatctagaac aagtttgtac aaaaagttga acgagaaacg taaaatgata
      961 taaatatcat atattaaatt agattttgca taaaaaacag actacttaat actgtaaaac
     1021 acacatatgc
//
