LOCUS       sequence-72994-e         997 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Contributes the coding sequence for the mKate2 fluorescent protein 
            as the 5'-module during MultiSite Gateway-cloning of chimeric cDNAs 
            encoding three-part fusion proteins..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 997)
  AUTHORS   Buj R, Iglesias N, Planas AM, Santalucia T
  TITLE     A plasmid toolkit for cloning chimeric cDNAs encoding customized 
            fusion proteins into any Gateway destination expression vector.
  JOURNAL   BMC Mol Biol. 2013 Aug 20;14(1):18. doi: 10.1186/1471-2199-14-18.
  PUBMED    23957834
REFERENCE   2  (bases 1 to 997)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "BMC Mol 
            Biol."; date: "2013-08-20"; volume: "14(1):18. doi"; pages: " 
            10.1186/1471-2199-14-18"
COMMENT     Sequence Label: pDONR P4-P1R-mKate2
FEATURES             Location/Qualifiers
     source          1..997
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    19..114
                     /gene="mutant version of attL"
                     /label=attL4
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     regulatory      117..126
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             123..818
                     /codon_start=1
                     /product="monomeric far-red fluorescent protein (Shcherbo 
                     et al., 2009)"
                     /label=mKate2
                     /note="mammalian codon-optimized"
                     /translation="MVSELIKENMHMKLYMEGTVNNHHFKCTSEGEGKPYEGTQTMRIK
                     AVEGGPLPFAFDILATSFMYGSKTFINHTQGIPDFFKQSFPEGFTWERVTTYEDGGVLT
                     ATQDTSLQDGCLIYNVKIRGVNFPSNGPVMQKKTLGWEASTETLYPADGGLEGRADMAL
                     KLVGGGHLICNLKTTYRSKKPAKNLKMPGVYYVDRRLERIKEADKETYVEQHEVAVARY
                     CDLPSKLGHR"
     misc_feature    831..896
                     /label=MCS
                     /note="multiple cloning site"
     protein_bind    909..933
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
ORIGIN
        1 acgctaccat ggagctccaa ataatgattt tattttgact gatagtgacc tgttcgttgc
       61 aacaaattga taagcaatgc ttttttataa tgccaacttt gtatagaaaa gttgccgcca
      121 ccatggtgag cgagctgatt aaggagaaca tgcacatgaa gctgtacatg gagggcaccg
      181 tgaacaacca ccacttcaag tgcacatccg agggcgaagg caagccctac gagggcaccc
      241 agaccatgag aatcaaggcg gtcgagggcg gccctctccc cttcgccttc gacatcctgg
      301 ctaccagctt catgtacggc agcaaaacct tcatcaacca cacccagggc atccccgact
      361 tctttaagca gtccttcccc gagggcttca catgggagag agtcaccaca tacgaagacg
      421 ggggcgtgct gaccgctacc caggacacca gcctccagga cggctgcctc atctacaacg
      481 tcaagatcag aggggtgaac ttcccatcca acggccctgt gatgcagaag aaaacactcg
      541 gctgggaggc ctccaccgag accctgtacc ccgctgacgg cggcctggaa ggcagagccg
      601 acatggccct gaagctcgtg ggcgggggcc acctgatctg caacttgaag accacataca
      661 gatccaagaa acccgctaag aacctcaaga tgcccggcgt ctactatgtg gacagaagac
      721 tggaaagaat caaggaggcc gacaaagaga cctacgtcga gcagcacgag gtggctgtgg
      781 ccagatactg cgacctccct agcaaactgg ggcacagagg tggaggaggt tccggactca
      841 gatctcgagc tcaagcttcg aattctgcag tcgacggtac cgcgggcccg ggatccaccg
      901 gatctagaac aagtttgtac aaaaaagttg aacgagaaac gtaaaatgat ataaatatca
      961 atatattaaa ttagattttg cataaaaaac agactac
//
