LOCUS       sequence-72996-e         837 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Contributes the coding sequence of a V5-6xHis epitope tags cassette 
            as the 3'-module during MultiSite Gateway-cloning of chimeric cDNAs 
            encoding three-part fusion proteins..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 837)
  AUTHORS   Buj R, Iglesias N, Planas AM, Santalucia T
  TITLE     A plasmid toolkit for cloning chimeric cDNAs encoding customized 
            fusion proteins into any Gateway destination expression vector.
  JOURNAL   BMC Mol Biol. 2013 Aug 20;14(1):18. doi: 10.1186/1471-2199-14-18.
  PUBMED    23957834
REFERENCE   2  (bases 1 to 837)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "BMC Mol 
            Biol."; date: "2013-08-20"; volume: "14(1):18. doi"; pages: " 
            10.1186/1471-2199-14-18"
COMMENT     Sequence Label: pDONR P2R-P3-V5-6xHis
FEATURES             Location/Qualifiers
     source          1..837
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    complement(54..177)
                     /gene="mutant version of attR"
                     /label=attR2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     CDS             201..242
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     CDS             252..269
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     protein_bind    complement(274..369)
                     /gene="mutant version of attL"
                     /label=attL3
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(406..425)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(407..425)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(430..446)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(430..446)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(548..567)
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     primer_bind     complement(626..645)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
ORIGIN
        1 cagctctaga gctcgaattc tacaggtcac taataccatc taagtagttg gttcatagtg
       61 actgcatatg ttgtgtttta cagtattatg tagtctgttt tttatgcaaa atctaattta
      121 atatattgat atttatatca ttttacgttt ctcgttcaac tttcttgtac aaagtggacg
      181 gtaccgagct cggatccgaa ggtaagccta tccctaaccc tctcctcggt ctcgattcta
      241 cgcgtaccgg tcatcatcac catcaccatt gaacaacttt attatacaaa gttggcatta
      301 taaaaaagca ttgcttatca atttgttgca acgaacaggt cactatcagt caaaataaaa
      361 tcattatttg gagctccatg gtagcgttaa cgcggccgcg atatccccta tagtgagtcg
      421 tattacatgg tcatagctgt ttcctggcag ctctggcccg tgtctcaaaa tctctgatgt
      481 tacattgcac aagataaaaa tatatcatca tgaacaataa aactgtctgc ttacataaac
      541 agtaatacaa ggggtgttat gagccatatt caacgggaaa cgtcgaggcc gcgattaaat
      601 tccaacatgg atgctgattt atatgggtat aaatgggctc gcgataatgt cgggcaatca
      661 ggtgcgacaa tctatcgctt gtatgggaag cccgatgcgc cagagttgtt tctgaaacat
      721 ggcaaaggta gcgttgccaa tgatgttaca gatgagatgg tcagactaaa ctggctgacg
      781 gaatttatgc ctcttccgac catcaagcat tttatccgta ctcctgatga tgcatgg
//
