LOCUS       sequence-72997-e        1036 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Contributes the coding sequence of a V5-6xHis epitope tags cassette 
            as the 5'-module during MultiSite Gateway-cloning of chimeric cDNAs 
            encoding three-part fusion proteins..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1036)
  AUTHORS   Buj R, Iglesias N, Planas AM, Santalucia T
  TITLE     A plasmid toolkit for cloning chimeric cDNAs encoding customized 
            fusion proteins into any Gateway destination expression vector.
  JOURNAL   BMC Mol Biol. 2013 Aug 20;14(1):18. doi: 10.1186/1471-2199-14-18.
  PUBMED    23957834
REFERENCE   2  (bases 1 to 1036)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "BMC Mol 
            Biol."; date: "2013-08-20"; volume: "14(1):18. doi"; pages: " 
            10.1186/1471-2199-14-18"
COMMENT     Sequence Label: pDONR P4-P1R-V5-6xHis
FEATURES             Location/Qualifiers
     source          1..1036
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    21..116
                     /gene="mutant version of attL"
                     /label=attL4
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     regulatory      119..128
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             149..190
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     CDS             200..217
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     protein_bind    218..342
                     /gene="mutant version of attR"
                     /label=attR1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(414..433)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(415..433)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(438..454)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(438..454)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(556..575)
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     primer_bind     complement(634..653)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
ORIGIN
        1 ttacgctacc atggagctcc aaataatgat tttattttga ctgatagtga cctgttcgtt
       61 gcaacaaatt gataagcaat gcttttttat aatgccaact ttgtatagaa aagttgccgc
      121 caccatgggt accgagctcg gatccgaagg taagcctatc cctaaccctc tcctcggtct
      181 cgattctacg cgtaccggtc atcatcacca tcaccataca agtttgtaca aaaaagttga
      241 acgagaaacg taaaatgata taaatatcaa tatattaaat tagattttgc ataaaaaaca
      301 gactacataa tactgtaaaa cacaacatat gcagtcacta tgaaccaact acttagatgg
      361 tattagtgac ctgtagaatt cgagctctag agctgcaggg cggccgcgat atcccctata
      421 gtgagtcgta ttacatggtc atagctgttt cctggcagct ctggcccgtg tctcaaaatc
      481 tctgatgtta cattgcacaa gataaaaata tatcatcatg aacaataaaa ctgtctgctt
      541 acataaacag taatacaagg ggtgttatga gccatattca acgggaaacg tcgaggccgc
      601 gattaaattc caacatggat gctgatttat atgggtataa atgggctcgc gataatgtcg
      661 ggcaatcagg tgcgacaatc tatcgcttgt atgggaagcc cgatgcgcca gagttgtttc
      721 tgaaacatgg caaaggtagc gttgccaatg atgttacaga tgagatggtc agactaaact
      781 ggctgacgga atttatgcct cttccgacca tcaagcattt tatccgtact cctgatgatg
      841 catggttact caccactgcg atccccggaa aaacagcatt ccaggtatta gaagaatatc
      901 ctgattcagg tgaaaatatt gttgatgcgc tggcagtgtt cctgcgccgg ttgcattcga
      961 ttcctggttg gtaatgtcct tttaacagcg atcgcgtatt tcgtctcgct caggcgcaat
     1021 cacgaatgaa taacgg
//
